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(i) The age of bacterial culture should not be more than 24 h.
At older age cell loses Gram positivity and will appear as Gram negative.
(ii) Application of heat during the fixation of smears is another important step. Too much heating during this step will lead to loss in, Gram positiveness.
(iii) Overcrowding of cells in smear also affects the result, due to improper decolourization.
(iv) Staining reagents should be freshly prepared.
(v) In Gram staining decolourizing step is very important. To obtain satisfactory differentiation, the nature and the exposure time of decolourizing agent should be standardized with the material to be stained. Acetone alone is more powerful decolourizing agent than ethanol.
(vi) It is also important not to allow a .bacterial smear to dry.
There are many variations of original Gram staining procedure
Wiki User
∙ 13y ago
Wiki User
∙ 14y agothe important factor which effect gram stain reactions is reaction time i.e. if the timing of dyes to be implied like meythlene blue and also timing of iodine expousre effects straining procedure then it comes to the concentraion of dyes which effect gram stain reaction
Wiki User
∙ 9y agoUsing actively growing cells while old cells lose their ability to hold the stain. Prepare thin smears and adjust decolorization time when cells are crowded they resist decolorization. Bacteria in thin smears decolorize faster than thick smears. Avoid overheating cells. Don't rinse too long water is a decolorize. Gram positivity is a characteristic that can be lost.
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The major criteria used in placing bacteria into different groups is based on differences in?
Biologists separate bacteria into two groups based on their reaction with the gram stain as Gram Positive and Gram Negative bacteria.
What does a gram reaction of a cell refer to?
the Gram reaction is based on the structure of the bacterial cell wall. In Gram-positive bacteria, the dark purple crystal violet stain is retained by the thick layer of peptidoglycan which forms the outer layer of the cell. In Gram-negative bacteria, the thin peptidoglycan layer in the periplasm does not retain the dark stain, and the pink safranin counter stain stains the peptidoglycan layer. In other word,the gram reaction refers to how the cells reacts to the gram-staining process.
What is schmorl's stain principle?
Melanin will reduce ferricyanide to ferrocyanide producing a blue coloured compound in the presence of ferric salts (Schmorl's reaction)
Which step of a gram stain can be omitted without affecting determination of the reaction?
you can omit the last step - the safranin counterstain
What is gram stain reaction for Borrelia Burgdoferi?
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
When a substance dissolves it leaves a stain which in science is called what?
A stain? Could be a salt: an ionic compound formed from the reaction between an acid and a base.
Does the liquid inside a glow stick stain clothes or hurt wood floors?
Yes there is a pigment in the stick after the chemical reaction & it will stain floors & textiles
Anyone have an allergic reaction to using Cabot Australian Timber Oil stain?
Just stained s door, and no reaction at all.
How do rags with oil based wood stain spontaneously catch fire?
the stain and the rag create a chemical reaction that causes it to rot and decompose very quiclky. this chemical reaction creates heat, and if it gets hot eanough, fire.
What is a three letter word for stain?
dye
What are the Factors That Affect Digestion in the stomach?
gastric ulcer may be also afect digestion main reason may be non hygienic foods Even MENTAL STRESS AND STAIN also may be a reason.
What is the gram stain reaction of strep throat?
Streptococcus pyogenes is gram positive bacteria.
Where is steel used in Rusting out how acids affect the rate of corrosion?
stain
What is the different reaction of Gram-negative and Gram-positive bacteria to Gram's stain testing?
Gram-positive bacteria which take up the stain turn purple, while Gram-negative bacteria which do not take up the stain turn red.
The major criteria used in placing bacteria into different groups is based on differences in?
Biologists separate bacteria into two groups based on their reaction with the gram stain as Gram Positive and Gram Negative bacteria.
How would the pH of the stain methylene blue affect the staining of bacteria?
the ph of the stain on the bacteria caused by methylene blue would not affect it a lot since all methylene blue is supposed to do is make it visible on the microscope for e.g.
Does heat affect stain when removing them eg. using hot water to remove a stain?
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