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Reagents of Osazone test are 1. Phenylhydrazine mixture consisting of phenyl-hydrazine and anhydrous sodium citrate 2. Orignal solution containing a carbohydrate

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Q: What Reagents are used in osazone test?
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What is the importance of phenylhydrazine test?

The phenylhydrazine test is also known as the Osazone test. The phenylhydrazine test is used for reducing the amounts of carbohydrates, momosacchstides, and disaccharides.


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Osazone test for carbohydrates?

osazone test is a carbohydrate specific reaction in which carbs react with phenylhydrazine to form osazones i.e. crystals of specific shape. thus the test confirms the presence of a particular sugar.Eg:for sucrose one gets a mixture of glucosazone and fructosazone on the basis of components of sucrose.


Why you used sodium acetate in osazone test?

Anhydrous sodium acetate is added to buffer the phenylhydrazine HCl so that it may function properly. Regards mawaisnawaz@hotmail.com


Why should there be an order of addition of the reagents in the sakaguchi's test?

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3reasons why the osazone test can be used in the identification of sugars?

1.due to their characteristic crystalline appearance 2.due to their definite melting point 3.due to the length of time for the crystals to form


3 reasons why the osazone test can be used in identification of sugars?

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