Microbiology | Genetics

What are degradative plasmid?

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degradative plasmids are types of plasmids present in certain bacterias such as pseudomonas putida which impart the ability of degrade xenobiotic compounds such as salicylic acid, 2-4D etc.

there are 3 such plasmids-

1)CAM plasmid- which degrades camphor.

2)XYL ,, - ,, ,, xylene.

3)NAH ,, - ,, ,, napthalene.

in addition to this CAM also has octane degrading OCT part & NAH has salicylic acid degrading SAL part.

What are the degradative plasmids and what are their properties?

it is a plasmid which utilizes secondary metabolites..........eg.P.putida

Do degradative reactions cause death?

Degradative reactions can cause cellular death. If the resulting factor is not treated appropriately the death of the organism can occur.

What does degradative reaction do?

The breakdown of an organic compound

When is a plasmid considered a recombinant plasmid?

When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.

Example of plasmid?

F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens.

Is 2H2O O2 synthetic or degradative reaction?

An example of spontaneous decomposition is that of hydrogen peroxide, which will slowly decompose into water and oxygen: : 2H2O2 → 2H2O + O2 thus it would be a degradative reaction

What is the function of the Ti plasmid?

what is the function of the plasmid

What is the difference between plasmid and bacteriophage lambda?

bacteriophage lambda is a better vector than plasmid as plasmid is small

Is expression vector a plasmid?

yes a expresssion vector can be a plasmid

What is meant by curing of plasmid DNA?

Curing a bacteria of plasmid DNA means getting rid of the plasmid so that the cells no longer have it.

A plasmid is a type of?

Plasmid is extrachromosomal DNA capable of self replication.

What occurs first in the production of a recombinant plasmid?

Isolation of a plasmid from a bacterium

What is the function of a plasmid function?

A plasmid is a circle of DNA that bacteria can absorb into the cell

What is a self-transmissible plasmid?

A plasmid which encodes genes for its own transfer.

What is a synonym for plasmid?

A plasmid is essentially extra-chromosomal self-replicating DNA

How many plasmid slots can you have in BioShock 2?

You can have a maximum of 8 plasmid slots.

Do mammalian cell has plasmid DNA?

Plasmid is the property of prokaryotic cell i.e. of bacteria generally no mamalian cell have plasmid but ya there can be chances to have lillte part of DNA sequence to be simillar that of plasmid DNA sequence in prokaryotes

How are plasmid classification on their property?

Plasmids are classified as: 1. F plasmid 2. R plasmid 3. Col plasmid F plasmids for fertility factor, it transfers its plasmid to the non fertile making it fertile. R plasmid for certain antibiotic resisitivity..for eg, ampicillin resistance. Col are certain proteins which when produced doesnt let other organisms to invade its cell.

Puc18 is a vector or a plasmid?

Puc18, i believe, is both. It is a plasmid vector. Plasmid is a more descriptive as it is a type of vector. Similarly to how a cheetah is both a mammal and an animal.

What is a multicopy plasmid?

Every plasmid has a copy number that reflects the average number of copies of a certain plasmid inside a host cell(usually a bacterial cell). So a multicopy plasmid, exist in multiple copies in any given bacteria. It is believed that the higher the copy number is, the more efficient the plasmid is at replicating itself.

What is a helper plasmid?

A helper plasmid is one that allows for the beginning of replication and transfer of other plasmids from a donor to a recipient. Without a helper plasmid, transposons will not be expressed in the recipient.

Role of tris edta glucose in plasmid isolation?

role of tris-chloride in plasmid isolation

Does an f strain donates a plasmid during conjugation?

Yes, it donates a plasmid to the F negative cell where that plasmid reproduces and makes it own strand in the F negative cell.

Why is ampicillin added to the pound medium?

Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means…

What do you call a population of cells carrying a desired plasmid?

A plasmid is a small molecule of DNA that replicate independently within the cell. A population of cells carrying a desired plasmid is called a clone.