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What are Tet and Amp, is it an antibiotic or a gene?

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Susanna Eichmann

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Q: What are Tet and Amp, is it an antibiotic or a gene?
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What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?

The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection


Antibiotic in gene cloning?

If antibiotic resistance is added to the gene being cloned, antibiotics can be used to isolate the transformed bacteria (ones with the gene being cloned) by killing off all non-transformed bacteria, that don't have the antibiotic resistance. There is a chance that the non-transformed bacteria can mutate to develop antibiotic resistance.


Is it true cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?

false


What can gene for antibiotic resistance be used for?

genetic marker


True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?

false


A gene for antibiotic resistance may be used as?

Genetic Marker


What is an extra loop of DNA that contains antibiotic resistance what gene?

Plasmid


How are cells screened to determine if the recombinant DNA was taken up?

Usually recombinant DNA is packaged in a plasmid that contains a marker gene. This marker can be an antibiotic resistance gene (NPTII for Kanamycin) or a gene that enables the plant to synthesise an amino acid. For antibiotic resistance the cells are grown on a medium that contains the antibiotic. The ones that grow have the marker gene. Sometimes the cells are transformed with a mixture of plasmids, some with the target gene and some without. The LAC-operon is used to select the cells that have the gene inserted. The gene-insertion inactivates the LAC-Z gene. Cells grown on X-gal plates will be blue, unless there's a transgene present. So white colonies have the transgene.


How can specialized transduction contribute to the transfer of antibiotic resistance genes in a bacterial population?

The prophage takes an antibiotic resistance gene with it and is packaged with the newly synthesized viral DNA.


Why are plasmids pieces of DNA?

i think each plasmid piece codes for a specific function. for example antibiotic resistance shown by antibiotic sensitive cell after a piece of plasmid that is antibiotic resistant gene recombine with cell DNA.


What one plate would you first inspect to conclude that the transformation occurred successfully Why?

If you transform bacteria with a plasmid containing a selection marker (such as an antibiotic resistance gene) and plate the transformed bacteria on a plate suited for selecting for plasmid-containing bacteria (such as a plate containing an antibiotic that only those bacteria with antibiotic resistance can survive), then simply inspecting whether colonies are present on the plate will suffice in determining whether the transformation succeeded. If no colonies are found, that means no bacteria got the antibiotic resistance gene on the plasmid and the transformation was unsuccessful. If some colonies are found, that means some bacteria contain the plamis containing the antibiotic resistance gene and those colonies can the transformation was successful.


What is the importance of Tet?

Tet is the time for tradition and family. All members in the family gather on Tet holiday.