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What are the advantages and limitations of column chromatography over thin layer chromatography?
Wiki User
∙ 9y ago
TLC. The mobile phase is a liquid, the stationary phase is a solid. Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase.
GLC. The mobile phase ia s gas, the stationary phase is a liquid on a solid support. same concept as TLC. useful for seperating gases by their affinities to the stationary phase...the mobility can then be compared to known compounds for possible identification.
Wiki User
∙ 11y ago
Wiki User
∙ 9y agoOne difference between column and thin layer chromatography is that in column chromatography the stationary phase is powdered adsorbent placed in a vertical glass column. In thin lay chromatography, the stationary phase is also powdered adsorbent, but it is fixed to a plate made from glass, aluminum, or plastic.
Wiki User
∙ 12y agothin layer chromatography uses alumna spread thinly on aluminum plates which can be made to any size that is needed,paper chromatography as the name suggests uses paper as its stationary phase and its applications are somewhat limited when compared to other forms of chromatography.the only time ive ever used paper chromatography was in the separation of ink consitiuants about 5 years ago
Answer:
Thin layer Chromatography uses silica, alumina & its compositions as a stationary phase while in paper chromatography, paper is being used as a stationary phase (in direct thinking). However, paper is used to support but it's not considered the stationary phase, which in this case, is water. The cellulose molecules in the paper can absorb water from the atmosphere and it is...this water + water present in the solvent that acts as the stationary phase.
Hope this help...
Wiki User
∙ 9y agoColumn chromatography can be scaled for a project. It is useful to separate and purify a reaction mixture, however it can take a long time. Thin layer chromatography is cheap and fast. However, it handles only very small amounts of compounds and is not good for uses outside of initial analysis.
Wiki User
∙ 12y agoChromatography is a technic where separation of molecules is carried by using chromosorbs packed in a column.
Ion Chromatography is a technic where separation is achievd by using ion chromosorbs packed in a column.
Adsorption chromatography is a technic where separation is achieved by using suitable chromosorbs packed in a column where respective molecules will stick externally to chromosorbs.
Wiki User
∙ 13y agoOne is faster and more flexible, the other is a bit heavier
Wiki User
∙ 10y agoi do need a complete logical answer
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Difference Rf values for monosaccarides and disaccharides?
Monosaccarides contain fewer polar covalent glycosidic bonds than polysaccharides and therefore move further up during thin layer chromatography and column chromatography.
What is the retardation factor?
In chemical chromatography, it is a measure of the relative mobility of components of a mixture through a stationary phase while experiencing the forces of a mobile eluent phase, based on relative intermolecular attractive forces and molecular size. In thin layer chromatography, is it the ratio of distance travelled by a component compared to the distance travelled by the eluent front from the point of contact with the mixture. In column chromatography, it is the fraction of the component in the mobile phase at equilibrium. By comparison, in gas chromatography, relative retention times on the stationary phase are measured and compared for the mixture components.
Which chromatographic process has minimal equipment and space requirements?
(TLC) Thin-Layer Chromatography
What technique would separate a solution with many components?
Fractional distillation is only useful if the components are liquid, and the separation is based on boiling point. Another, more applicable method would be chromatography, either thin layer, paper, or column.
What are the significance of Retention factor values?
Rf Values determine the solubility of a substance with respect to a certain solvent. It also determines the affinity of the solute to the solvent (greater Rf=greater affinity of solute to the solvent)
What are the Advantages of column chromatography over thin layer chromatography?
One is faster and more flexible, the other is a bit heavier
What are the differences between column and thin layer chromatography?
In chromatographic terms, TLC has great advantages over the other chromatography modes, such as Liquid Chromatography (LC), Column Chromatography (CC), Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC).TLC's advantages are: (1) the ability to perform multiple analyses simultaneously; (2) speed and ease for scouting separation conditions, such as optimum solvent mixtures.
Difference Rf values for monosaccarides and disaccharides?
Monosaccarides contain fewer polar covalent glycosidic bonds than polysaccharides and therefore move further up during thin layer chromatography and column chromatography.
What are 3 types of chromatography?
1. thin -layer chromatography 2. gas chromatography 3. liquid chromatography
Are paper and thin layer chromatography types of planer chromatography?
yes.
What has the author Bernard Fried written?
Bernard Fried has written: 'Thin-layer chromatography' -- subject(s): Thin layer chromatography
What technique is used to separate salt and sugar?
Any of the three types of chromatography (column , thin - layer or paper) can be used to separate the salt from sugar and vice-verse !
How is paper chromatography used in everyday life?
it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.
What is the name of the method used to separate dyes?
Chromatography is the method used to separate a mixture of dyes or inks.
How does pH affect thin layer chromatography?
The Different Types of Chromatography There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography. Liquid Chromatography is used in the world to test water samples to look for pollution in lakes and rivers. It is used to analyze metal ions and organic compounds in solutions. Liquid chromatography uses liquids which may incorporate hydrophilic, insoluble molecules. Gas Chromatography is used in airports to detect bombs and is used is forensics in many different ways. It is used to analyze fibers on a persons body and also analyze blood found at a crime scene. In gas chromatography helium is used to move a gaseous mixture through a column of absorbent material. Thin-layer Chromatography uses an absorbent material on flat glass or plastic plates. This is a simple and rapid method to check the purity of an organic compound. It is used to detect pesticide or insecticide residues in food. Thin-layer chromatography is also used in forensics to analyze the dye composition of fibers. Paper Chromatography is one of the most common types of chromatography. It uses a strip of paper as the stationary phase. Capillary action is used to pull the solvents up through the paper and separate the solutes.
What has the author F Geiss written?
F. Geiss has written: 'Fundamentals of thin layer chromatography (planar chromatography)'
What is the mechanism of separation in thin layer chromatography?
i think mechanism is that,,thin layer chromatography is basically separation of mixture,and sample might adsorb on silics surface.here is adsorption mechanism
