0
Studies are inconclusive that ethidium bromide, an inhibitor for cell growth, is not shown to be toxic in humans, but the metabolites may exude mutagenic characteristics.
Wiki User
∙ 10y ago
Add your answer:
Earn +20 pts
Why dont you use ethidium bromide staining for non denaturing PAGE gels?
Because PAGE gels are used to look a protein and ethidium bromide only stains double stranded nucleic acids.
How does ethidium bromide interact with double stranded DNA Does it increase or remove supercoiling?
when ethidium ion intercalates between two dna base pairs in a circular dna it causes the dna to unwind by 26 degrees, thereby decreasing twist and increasing writhe. in a circular dna which is negatively supercoiled, if ethidium is added it will become relaxed and if more ethidium is added dna becomes positively supercoiled
What is the role of Ethidium bromide in plasmid isolation?
Ethidium bromide is an intercalator, meaning it inserts itself between the base pairs of DNA. Linear DNA pieces like the genomic DNA fragments bind more ethidium bromide than the circular plasmid DNA. The solution is placed into a tube that is spun extremely fast (roughly 50,000 revolutions per minute) in an ultracentrifuge for about a day. During this time the cesium chloride forms a gradient of lower density at the top of the tube and higher density at the bottom. The genomic and plasmid DNA form tight bands in this gradient. Since the plasmid DNA binds less ethidium bromide it is more dense and is located lower in the tube than the genomic DNA. RNA forms a separate band at the bottom of the tube. These three bands can be visualized by UV light.
What is the DNA staining solution called?
There are a few staining methods that can be used. Common are methylene blue, ethidium bromide, fast blast, sybr safe.
While loading total RNA in farmaldehide gel a streek pattern is showing above the well in opposite direction of RNA movement and also band is very light in gel WHY?
its ethidium Bromide that's moving out!!!!! check with the amt of rna loaded!and its quite possible the isolation is bad... how is the o.dAnswerits ethidium Bromide that's moving out!!!!! check with the amt of rna loaded!and its quite possible the isolation is bad... how is the o.d
How does ethidium-bromide stain DNA?
Ethidium bromide binds with DNA and slips in between its hydrophobic base pairs and stretches the DNA fragment, removing water molecules from the ethidium cation. The result of this dehydrogenation is an increase in fluorescence of the ethidium (as well as the cell).
What does ethidium bromide stain in a cell?
Ethidium bromide is a chemical that is able to intercalate (fit between the bases) between the bases of double stranded DNA strands
Why dont you use ethidium bromide staining for non denaturing PAGE gels?
Because PAGE gels are used to look a protein and ethidium bromide only stains double stranded nucleic acids.
What has the author S Chaudhuri written?
S. Chaudhuri has written: 'Radiation studies on ethidium bromide'
Why ethidium bromide is carcinogenic?
The purpose of ethidium bromide is as an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. When exposed to ultraviolet light, it will fluoresce with an orange color, intensifying almost 20-fold after binding to DNA. Hence it is useful in visualizing DNA. Unfortunately, ethidium bromide does not distinguish between what DNA it binds to, whether it be ours or our sample's. Hence ethidium bromide is a mutagen, suspected carcinogen and at high concentrations is irritating to the eyes, skin, mucous membranes and upper respiratory tract. Ethidium bromide acts as a mutagen because it intercalates into double stranded DNA, thereby deforming the molecule. This is believed to block or trip biological processes occurring on DNA, like DNA replication and transcription.
Why cybersafe replaced ethidium bromide?
Because is it less hazardous while still having good results
What are the common stains that is used after DNA electrophoresis?
Ethidium bromide and coomassie blue are some stains that can be used in DNA electrophoresis.
How does ethidium bromide interact with double stranded DNA Does it increase or remove supercoiling?
when ethidium ion intercalates between two dna base pairs in a circular dna it causes the dna to unwind by 26 degrees, thereby decreasing twist and increasing writhe. in a circular dna which is negatively supercoiled, if ethidium is added it will become relaxed and if more ethidium is added dna becomes positively supercoiled
What are the environmental consequences that may result over time if the warning about disposal of ethidium bromide is frequently?
increased rate of cancer near the landfills
What is the function of ethidium bromide in DNA extraction?
EtBr interculates into DNA and when exposed to UV light, it causeses DNA to nick and therefore uncoil
What is the role of Ethidium bromide in plasmid isolation?
Ethidium bromide is an intercalator, meaning it inserts itself between the base pairs of DNA. Linear DNA pieces like the genomic DNA fragments bind more ethidium bromide than the circular plasmid DNA. The solution is placed into a tube that is spun extremely fast (roughly 50,000 revolutions per minute) in an ultracentrifuge for about a day. During this time the cesium chloride forms a gradient of lower density at the top of the tube and higher density at the bottom. The genomic and plasmid DNA form tight bands in this gradient. Since the plasmid DNA binds less ethidium bromide it is more dense and is located lower in the tube than the genomic DNA. RNA forms a separate band at the bottom of the tube. These three bands can be visualized by UV light.
What is the DNA staining solution called?
There are a few staining methods that can be used. Common are methylene blue, ethidium bromide, fast blast, sybr safe.
