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How is Benedict's test used to detect reducing and non-reducing sugars?
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∙ 8y ago
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Mariam Iyiola
Mossie Lueilwitz
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∙ 12y agoprecautions for simple sugar
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What compound can be oxidized by both Benedicts solution and Tollens reagent?
All the compounds having free aldehyde group as reducing sugars.
How do you differentiate between reducing and non reducing sugar?
Add benedicts solution to the sample you are testing, heat gently for 5 minutes, and if reducing sugars are present, the solution turns red (if concentration of reducing sugars is high) and if its not as high it could turn green-yellow-brown ish the closer to red, the higher the concentration of reducing sugars. it will stay blue if none are present
Would glucose solution produce a positive Benedicts test?
No, Benedicts reagent will show positive results if the carbohydrate is a reducing sugar. You will know if it is positive if the sample will turn from blue to green then to orange when you are cooling the solution, which is the last step when you are performing the benedicts test for carbohydrates.
What is the difference between reducing and non reducing sugar?
The Fehling's and the Benedict's Test are the just two of the many tests conducted in identifying reducing and non-reducing sugars. Reducing sugars like the monosaccharides can reduce cupric hydroxide from the reagents used. This is because the reducing sugars have a free oH group at their anomeric carbon that can cause the reduction of mild oxidizing agents like fehling and Benedict solution.In non reducing sugars this oH is involved in glycosidic bond formation.
When a non reducing sugar is tested with Benedict's reagent?
Non reducing sugars do not react with Benedict's reagent. After the test, sample without reducing sugars remains the same, blue.When reducing sugars are present in the sample, we can consider four results after the test is completed: a) green, low amount, that is 0.1 to 0.5% of reducing sugars in solution; b) yellow, low amounts of reducing sugars, 0.5 to 1.0%; c) orange, moderate content of reducing sugars, 1.0 to 1.5% of reducing sugars present; and c) brick red, large amount of reducing sugars in solution, 1.5 to 2.0%.
What are nonreducing sugars?
reducing sugars are those sugars which cannot donate electron and cannot reduce other solution
Would raffinose react with benedicts reagent?
Benedicts reagent tests for reducing sugars, so the question is, is raffinose a reducing sugar. Raffinose is a trisaccharide made up of glucose, fructose and galactose. It is not a reducing sugar because all of its anomeric carbons are bonded, so it will not react with benedicts reagent.
Which carbohydrates give a positive reaction with benedicts reagent?
all reducing sugars - monosaccharides like glucose and a few non-reducing disaccharides like maltose
What compound can be oxidized by both Benedicts solution and Tollens reagent?
All the compounds having free aldehyde group as reducing sugars.
How do you differentiate between reducing and non reducing sugar?
Add benedicts solution to the sample you are testing, heat gently for 5 minutes, and if reducing sugars are present, the solution turns red (if concentration of reducing sugars is high) and if its not as high it could turn green-yellow-brown ish the closer to red, the higher the concentration of reducing sugars. it will stay blue if none are present
What does a negative Benedicts test indicate?
A negative Benedict's test would indicate that there isn't any presence of reducing sugars in that particular substance.
Will hydrolysed dextrine give positive response to Benedict test?
Yes, it should. Benedicts test will be positive for reducing sugars, and since glucose is such a sugar, and would be a product of dextrin hydrolysis, you should get a positive result with Benedicts reagent.
Does DNSA detect polysacchrides?
It detects reducing sugars. DNSA -----> 3-A, 5-N SA
What is non-reducing sugar?
Non-absorbable sugars are also called sugar alcohols. They are sweet-tasting, but are not readily absorbed from the intestine into the body. Most are manufactured for commercial use. Examples are non-caloric sweeteners, like sorbitol and mannitol, which are often used in candies and gums. Non-absorbable sugars may be prescribed as laxatives.
When glycogen is added to benedicts reagent why would it remains blue?
Although glycogen is a carbohydrate, it is classified under POLYSACCHARIDES which are non-reducing sugars. Benedict's reagent is used to test for the presence of non-reducing sugars. The positive result for this is a brick-red precipitate or solution. It remained blue (which is negative) due to fact that ALL POLYSACCHARIDES (such as glycogen) ARE NON-REDUCING SUGARS > I'm a Nursing Student. >M.J.T.M.E.
What causes Benedict's reagent to turn red?
This is due to the reduction of the cupric (Cu2+) ions in Benedict's reagent to cuprous form (Cu1+) by reducing sugars, forming cuprous oxide (Cu2O), which is a brick red precipitate. This is why Benedict's test is a good way to detect reducing sugars.
Would glucose solution produce a positive Benedicts test?
No, Benedicts reagent will show positive results if the carbohydrate is a reducing sugar. You will know if it is positive if the sample will turn from blue to green then to orange when you are cooling the solution, which is the last step when you are performing the benedicts test for carbohydrates.
