What are the principles of microscopy?
principle of microscopy includes 2 important aspects:
1. resolution and resolving power- the resolving power of a
microscope is defined as the minimum distance by which 2 points
must be separated in order for them to be perceived as 2 separate
points rather than a fused image.
2. magnification- Linear (sometimes called lateral or
transverse) magnification refers to the ratio of image length to
object length measured in planes that are perpendicular to the
optical axis. A negative value of linear magnification denotes an
inverted image. Longitudinal
magnification denotes the factor by which an image increases in
size, as measured along the optical axis.