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What are well isolated colonies?

Updated: 8/10/2023
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15y ago

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Certain Isolation techniqiues are based on the concept that if an individual bacterial cell is separated from other cells and provided adequate space on a nutrient surface, it will grow into discrete mound of cells called a colony

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11y ago
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12y ago

You are basically asking how do animals evolve.

Say a group of birds found an island with no predators and a constant supply of food. The birds would never leave and over time, the birds hatched on the island would loose abilities they did not need such as fear and flight through minor 'mutations' and finally we would end up with birds like dodos.

Also, if predatory bird as an eagle were to find that island and start to prey on the first birds, each new generation of eagle would have minor 'mutations' and those with the best tactics of hunting and getting food would survive to pass on their abilities to its young until a completely new species develops.

This is the same for all life forms.

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15y ago

Well isolated colonies are colonies either on a streak plate or a slant test tube that are single structures of bacteria set apart from all the rest.

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Q: What are well isolated colonies?
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Why restreak isolated colony?

The isolated colonies for the new plate can be used to compare with the isolated colonies on the old plate. If the morphologies are the same, it can be assumed that pure culture is obtained.


Will isolated colonies of bacteria always be in the fourth sector on the streak plate?

No, of course it is possible that one could start out with an already diluted sample in the first step. So then perhaps in the third sector some isolated colonies may form, because the amount of bacteria have been diluted enough.


Does providencia bacteria ferment lactose?

No, it doesn't. After performing the Lactose fermentation test, the isolated colonies will turn yellow meaning that the test was negative.


Why use a streak plate to grow a bacterium?

You do a streak plate in order to get isolated colonies. If you inoculate into a slant, you have less surface area to work and less chance of getting isolated colonies. In broth, you'll definitely get growth but you won't know WHAT is growing. You go back into each quadrant (a little) with your loop in order to "dilute" the bacteria and get colonies. Quadrant 1 is pretty think (like a smear on the plate) but by the time you get to Quadrants 3 and 4, you should see more defined colonies and not just a film of bacteria.


Why is it important to start a bacterial culture with a single isolated colony?

It is important for someone who works with bacteria to have a genetically identical bacterial colony so they can test different things on one type of bacteria. Making observations on that one colony is valuable for visual research and identification of that microorganism.

Related questions

Why restreak isolated colony?

The isolated colonies for the new plate can be used to compare with the isolated colonies on the old plate. If the morphologies are the same, it can be assumed that pure culture is obtained.


What factor contributes to the difference in the appearance of isolated versus crowded colonies?

1. The factors that contribute to the differences in appearance of isolated vs crowed colonies are: 1. Availability of nutrients, where colonies are crowed the bacteria will be in competition for nutrients and because of this there can be a reduction of size and shape also growth can also be stunted whereas in isolated colonies the nutrients is readily available with no competition for nutrients. 2. space, isolated colonies have more space to grow as compared to crowded colonies and 3. If there is contamination in a crowded colony the texture will be very difficult to determine however texture can be viewed in isolated colonies so contamination is a factor.


What is essential for development of discrete isolated colonies?

broth medium


Why must have 4 part in streaking technique?

so that you can get isolated colonies in the last streak . . . As you streak contineously you inoculum quantity decreases . . there by when you reach the end of last streak you get separate and isolated colonies . .


What is essential for development of discrete isolated colonies microbiology?

broth medium


Will isolated colonies always be in the second sector on the streak plate?

No. It depends on the number of bacteria present in the initial sample. If the number of bacteria in the initial sample are limited, you may get isolated colonies in the first streak. If the number of bacteria in the sample are high, it may take several streaks before the sample is diluted to the point where isolated colonies are evident.


Did Parliament successfully isolated Boston from the rest of the colonies by passing the coercive acts?

well according to history parliament did pass the the coercive acts but it wasnt to isolate Boston from from the rest of the colonies. so i hope you learned something today.


Did the Parliament successfully isolated Boston from the rest of the colonies by passing the coercive acts?

well according to history parliament did pass the the coercive acts but it wasnt to isolate Boston from from the rest of the colonies. so i hope you learned something today.


Will isolated colonies of bacteria always be in the fourth sector on the streak plate?

No, of course it is possible that one could start out with an already diluted sample in the first step. So then perhaps in the third sector some isolated colonies may form, because the amount of bacteria have been diluted enough.


A tightly closed well-insulated vacuum flask is an example of which type of system?

isolated system


Definition for base isolated building?

Well...I would think that it is a building that's base is isolated i don't know you tell me


Why are koalas made how they are to live in were they do?

Koalas have adapted to changes in their environment as people build close to them, their colonies are getting smaller and more isolated.