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In a gram positive stain it is because the cell's cell wall is made up of peptioglycan
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Explain why crystal violet would not be useful in the negative stain technique?
Negative staining techniques are designed to stain everything BUT the bacterial cells. This allows us to see the cells unstained and helps us observe their morphology (how the cells are shaped and how they group together). The cells have an overall negative charge on their surface, so they naturally attract positive charges. Crystal Violet carries a positive charge, so it would adhere to the surfaces of the cells thus staining the cells (which is not what you want in a negative stain!)
What kind of stain are used to stain plant and animal cells?
Methylene blue stain is used to stain plant and animal cells.
Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
Why do all cells stain purple in the flagella stain but not in the gram stain?
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
What causes a stain to adhere to bacteria cells and why all colored dyes not necessarily useful for simple staining?
The causes a stain adheres to bacterial cells is the color-bearing ions (chromophores) and they might be positively charged (cationic), such as methylene blue, basic fuchsin, and crystal violet, because bacteria are negatively charged and anionic chromophores, such as eosin, will not stain bacteria because of the electrostatic repelling forces that are involved.
Explain why crystal violet would not be useful in the negative stain technique?
Negative staining techniques are designed to stain everything BUT the bacterial cells. This allows us to see the cells unstained and helps us observe their morphology (how the cells are shaped and how they group together). The cells have an overall negative charge on their surface, so they naturally attract positive charges. Crystal Violet carries a positive charge, so it would adhere to the surfaces of the cells thus staining the cells (which is not what you want in a negative stain!)
What color are gram negative cells?
Gram positive bacterial stain purple. They do not take up the counter stain.
Can you heat fix blood smear before staining for bacterial examination?
You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Green
What is the chemical composition of bacterial capsule?
It usually consists of polysaccharides,[1] but can be composed of other materials (e.g., polypeptide in B. anthracis). Because most capsules are water soluble[citation needed], they are difficult to stain using standard stains because most stains do not adhere to the capsule. For examination under the microscope, the bacteria and their background are stained darker than the capsule, which doesn't stain. When viewed, bacterial cells as well as the surface they are on, are stained dark, while the capsule remains pale or colorless and appears as a ring around the cell.
What kind of stain are used to stain plant and animal cells?
Methylene blue stain is used to stain plant and animal cells.
Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
Why do all cells stain purple in the flagella stain but not in the gram stain?
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
Why doesn't a negative stain colorize the cells in the smear?
A negative stain will stain the background with an acidic dye, such as Nigrosin. This procedure is used to demonstrate capsules. This technique brings the specimen off of the background for more adequate viewing purposes.
What would happen if the step involving Gram's iodine was inadvertently left out of the Gram staining procedure?
Gram staining refers to separating bacterial species into two groups, gram-positive and gram-negative, which defines the physical and chemical properties. Skipping a step in this process could result in a mixed group of the bacterial species.
