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It is crystal violet & stains all cells purple.
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∙ 12y ago
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Why gram staining procedure cannot stain endospores?
Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
What is determined of the Gram Stain?
A gram stain is a cow
Can a Gram stain a prion?
Protists are often stained using a silver stain, not a Gram stain.
What is secondary and counter stain?
counterstains are selected to be contrasting color so that the target of the primary stain can easily be differentiated on a contrasting background. This makes life easier, when, for example you need to count the number of nuclei in a smear, or number of gram positive bacteria in a mixed population.
Why does the counterstain not change the color of all of the cells is it because the primary stain repels or masks the secondary stain?
In a gram stain the primary stain is crystal violet. Iodine then sets that dye into the gram positive cells while alcohol washes out the crystal violet from the gram negative cells. Then safranin, which is the counterstain in a gram stain, is used to dye the rest of the bacteria. This is the example I can give you of why a counterstain does not change the look in all the cells. Though safranin stains all the cells, the gram positive cells that were dyed purple from crystal violet don't look pink - only the gram negative do.
What is the purpose of the crystal violet in the gram stain procedure?
Its the primary stain of the procedure. IT stains the Gram positive organisms
What is the purpose of crystal violet in the Gram's stain procedure?
Its the primary stain of the procedure. IT stains the Gram positive organisms
Does the gram stain procedure work for all bacteria?
Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.
Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
Safranin
Is B subtilis a gram positive or gram negative bacterium?
gram positive Exactly. When doing a gram stain on B. subtilis, this bacterium resists decolorization (keping the first stain and NOT taking on the color of the secondary stain). Therefore, this bacterium is gram (+).
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Why gram staining procedure cannot stain endospores?
Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
What is determined of the Gram Stain?
A gram stain is a cow
Why do all cells stain purple in the flagella stain but not in the gram stain?
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
Theories of principle of gram stain reactions?
Gram stain
What is the gram stain for cholera?
Gram Negative
Can a Gram stain a prion?
Protists are often stained using a silver stain, not a Gram stain.
