What did Watson need to consider when he worked out the DNA base pairing?
- Bond angles
- Bond lengths
- The location of hydrogen atoms
- Chargaff's pairs (although he didn't recognize them at the time)
Erwin Chargaff showed that the number of guanine units was equal to the number of cytosine units, and that the number of adenine was equal to the number of thymine. This hinted at a base pairing rule - but Chargaff did not explicitly state a rule. Watson and Crick later proposed the base pairing rule.
Watson and Crick came up with the base-pairing rule for nucleic acids using Chargaff's rule that in DNA the percentages of adenine and thymine are equal, and the percentages of guanine and cytosine are equal. Refer to the related link below. Maurice Wilkins also shared the Nobel Prize in 1962 for Physiology or Medicine along with Watson and Crick for coming up with the model for the structure of DNA.
Watson and Crick. Using cardboard cutouts they recognized the helical shape of DNA and the complimentary base pairing rule. That base A pairs with T and Base C pairs with G. They used information from Rosalind Franklin (who determined the width of DNA and the helical shape) and Chargoff (who discovered the equality rule). The structure of this model developed by Watson and Crick suggested a replication mechanism. Hope this helps!
What was wrong with watsons hypothesis that gene replication could be acheived if each base in the newly synthesized chain hydrogen hydrogen bonded to an identical base?
Sir James Watson and Sir Francis Crick first described the molecular structure of DNA correctly. They not only mentioned the fact that DNA is a helical structure, but also described how the constituting molecules are arranged in the helix. The base-pairing, sugar-phosphate backbone were first described by Watson and Crick.
Base pairing refers to the pairing of complimentary nitrogen bases, either during DNA replication, or transcription and translation. In DNA, the bases adenine and thymine pair together, and guanine and cytosine pair together. In RNA, the base uracil takes the place of the base thymine. The bases that pair together are said to be complimentary to each other.
Base pairing refers to the process in which complimentary nitrogen bases pair with one another, in which adenine pairs with thymine and guanine pairs with cytosine. Base pairing is involved in DNA replication in that when each original strand of DNA unzips, new DNA nucleotides pair with their complimentary bases on the original strands, resulting in two identical molecules of DNA, each with an original strand and a new strand.
During transcription of RNA or DNA base pairing is essential to keep cell genetics consistent. In DNA the base pairing formula is what determines everything that is us all the way down to what proteins needed for cellular reproduction to immune functions. Uracil will replace Thiamine during RNA transcription. Mutation will occur if the base pair is changed.