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EDTA has high affinity towards divalent ions like Ca2+, Mn2+, Mg2+ which are cofactors for many active enzymes inside the cells. That includes nucleases which digests DNA molecules. Once the cell is disrupted, nuclear envelope goes off and the nuclear content comes into contact with the cellular content which is rich in nucleases. So the broken cell is treated with EDTA to chelate the ions so that nucleases loose their function and that we are able to get good yield of DNA.
Wiki User
∙ 15y ago
Wiki User
∙ 14y agoEDTA chelates divalent metals (primarily magnesium and calcium) which is co-factors for DNAse. In other words, EDTA inhibit DNase
Wiki User
∙ 12y agoIt inhibits nucleases leading to the rupture of cell wall and membrane.
Wiki User
∙ 11y agoIt chelates divalent cations which can catalyze RNA breakage
Wiki User
∙ 10y agoIt protects DNA or RNA from degradation.
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When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
Why EDTA is not dissolved in water?
EDTA is soluble in water.
Is sodium chromate the same as edta?
No. Edta is Ethylenediaminetetraacetic acid
What is tertrasodium edta?
Tertrasodium EDTA is a chelating and preservative agent.
Analogy for plasmid?
F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens.
Why ph8 is maintained during isolation of plasmid?
EDTA is dissolved only at pH8. EDTA serves as an important chelating agent to kill the contaminating DNAses. Also this is close to the physoological pH which is pH7.
Why ammonia is added in preparation of EDTA solution?
To maintain pH=10
Why is Ammonia solution added while preparing EDTA solution?
ammonia solution is added during the preparation of EDTA solution to increase the rate of dissolution of its disodium salt.
What is the Role of RNAase in plasmid preparation?
In plasmid isolation RNA behaves as an unwanted material so to separate it out RNAase is required which breaks down the RNA. This is done to get pure quality of the product.
You have propagated a vector in a non expression host and isolated the plasmid How can you confirm whether the plasmid that you got is exactly the vector which you propagated?
Check the size in agarose gel, extract it from the gel then purify it and grow it on selective plate.
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
What is the function of the Ti plasmid?
what is the function of the plasmid
Why EDTA is not dissolved in water?
EDTA is soluble in water.
What is the basicity of EDTA?
the basicity of EDTA is 4
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
What plasmid that can degrade toluic acid?
TOL plasmid
What is EDTA whole blood?
EDTA whole blood is whole blood that has been drawn into a tube with EDTA in it. The EDTA is added to transport samples and prevents the blood from clotting.
