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Answer:
The factors which affect the chromatography are:
Temperature
Pressure
Flow rate of mobile phase
Sample preparation
Wiki User
∙ 12y ago
Wiki User
∙ 14y agoin chromatography smaller particles than large protriens could be identified with progressive molecular mass,peptide sequences could be read from semiliquid aliquotes and direct aliquotes for various amino acids, simple carbohdrates n glycoproteins
Wiki User
∙ 13y agoTHERE ARE MANY FACTORS DEPENDING UPON THE TYPE OF ANALYSIS
such as column length, flow rate, gradient shape, temperature and buffer pH
Resolution depends not only on the column, but also on the mobile phase composition and flow-rate, the gradient rate and the size and composition of the peptides themselves.
Wiki User
∙ 14y ago- Temperature ramp rate
- Column length
- Carrier gas flow rate
- Film thickness
- Column internal diameter
- Ultra fast technology
Wiki User
∙ 12y agoVariation in:
number of plates
retention factor
selectivity factor
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What is the difference between flash chromatography and column chromatography?
Column chromatography, is a broad term for all column chromatography methods, but is also synonomous with Gravity fed methods. Flash chromotography refers specifically to a column in which the eluant (or mobile phase) is moved through the column under pressure (using a hand pump for small scale, or a pressurised gas for a larger scale), the name Flash is derived from how much faster it is to run a column under pressure than via gravity.
How do you separate the enzymes in column chromatography?
In column chromatography, the enzymes are made to pass through the column without occurrence of bubbles. These enzymes are obtained at the end of the process by slowly advancing through every column.
What is the column material in gel filtration chromatography?
the column is some strange scientist
How monomeric and polymeric bonding type affect separation in column chromatography?
The more monomers that are strung together, the slower they will move through the resin. This is a oversimplification as branches in the polymer can occur and they affect the migration as well.
What are the main applications of column chromatography?
Column chromatography is used in the lab and industry to isolate the compound that they want. Since some chemical reactions are not selective to the product you want, you have to get rid of the products you don't want. Sometimes column chromatography is the only way.
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What is the difference between flash chromatography and column chromatography?
Column chromatography, is a broad term for all column chromatography methods, but is also synonomous with Gravity fed methods. Flash chromotography refers specifically to a column in which the eluant (or mobile phase) is moved through the column under pressure (using a hand pump for small scale, or a pressurised gas for a larger scale), the name Flash is derived from how much faster it is to run a column under pressure than via gravity.
How do you separate the enzymes in column chromatography?
In column chromatography, the enzymes are made to pass through the column without occurrence of bubbles. These enzymes are obtained at the end of the process by slowly advancing through every column.
Are Adsorption and partition chromatography types of column chromatography?
Yes,both can performed in columns.
What is the column material in gel filtration chromatography?
the column is some strange scientist
What is the purpose of an alumina column?
Column chromatography is generally used as a purification technique: it isolates desired compounds from a mixture.Column chromatography is separated into two categories, depending on how the solvent flows down the column. If the solvent is allowed to flow down the column by gravity, or percolation, it is called gravity column chromatography. If the solvent is forced down the column by positive air pressure, it is called flash chromatography, a "state of the art" method currently used in organic chemistry research laboratories The term "flash chromatography" was coined by Professor W. Clark Still because it can be done in a "flash."
What is the importance of a mixed elution solvent in column chromatography?
In column chromatography, the stationary phase, a solid adsorbent, is placed in a vertical glass (usually) column and the mobile phase, a liquid, is added to the top and flows down through the column (by either gravity or external pressure). Column chromatography is generally used as a purification technique: it isolates desired compounds from a mixture.
What is sorbsil?
It is a column chromatography grade silica gel.
How monomeric and polymeric bonding type affect separation in column chromatography?
The more monomers that are strung together, the slower they will move through the resin. This is a oversimplification as branches in the polymer can occur and they affect the migration as well.
What are the Advantages of column chromatography over thin layer chromatography?
One is faster and more flexible, the other is a bit heavier
What are the differences between column and thin layer chromatography?
In chromatographic terms, TLC has great advantages over the other chromatography modes, such as Liquid Chromatography (LC), Column Chromatography (CC), Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC).TLC's advantages are: (1) the ability to perform multiple analyses simultaneously; (2) speed and ease for scouting separation conditions, such as optimum solvent mixtures.
What are the main applications of column chromatography?
Column chromatography is used in the lab and industry to isolate the compound that they want. Since some chemical reactions are not selective to the product you want, you have to get rid of the products you don't want. Sometimes column chromatography is the only way.
