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There will be too much of the sample for it to effectively separate over such a short distance. The result will be a splotchy streak instead of several separate spots after development.

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14y ago
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12y ago

there would be taililng and overlapping of spots during development which will cause problem in raeding the distance moved by spot

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12y ago

The Rf value will be very hard to determine.

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Q: What happen if the spots on TLC plate are made too small?
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