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What is Cut time in HPLC or GC?
Wiki User
2007-11-28 06:41:00
10 - 15 minutes
Related Questions
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Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time
compared to hplc. additionally, there is less compatibility issue
in setting an MS up to a GC than HPLC
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What are the advantages of HPLC over GC?
The time required for separations vary less for HPLC than GC.
High pressure is used in HPLC. Length of the column is small. GC is
used for only volatile compounds mainly and only 15% of organic
compounds are volatile but in HPLC both volatile and non volatile
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What is the difference between HPLC and GC?
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The principle difference between the hplc and gas chromatography?
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What analytic method is more preferrable betwee GC and HPLC?
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Retention time in Hplc?
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Retention time calculation for hplc?
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How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the
solvents used are less polar than the substrate in the HPLC column
(e.g. using hexane or dichloromethane with a silica HPLC column).
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Difference hplc assay and hplc purity and hplc potency?
HPLC purity :
It explains how pure our analyte is in the given mixture .It is
not related to the how much our analyte is in the given
mixture.
i.e Percentage of a our analyte with out impuritys in
HPLC.(Known or Unknown)
HPLC assay :
It explains how much is our analyte in the given mixture(The
content of our component in the given mixture).It is not related to
analyte purity.
HPLC potency :
It is measurement of our analyte how potent it is.
i.e Purity of our analyte with out all possible impuritys like
chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy
metals,sulphated ash ..etc
For example:
If we have a analyte of some X of purity 99.5%.
Prepare 20%,60% and 90% of solution of X.inject all these
solution in hplc.
For 20% solution you will get 99.5% purity and 20%
assay.
For 60% solution you will get 99.5% purity and 60%
assay
For 90% solution you will get 99.5% purity and 90%
assay.
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What is void time in hplc?
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Definition of standard in HPLC?
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What is the principal of HPLC?
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liqued chromatography
PRINCIPAL:the main principal of HPLC is adsorbtion the princepal
of separation is highly