GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
HPLC is advanced
The time required for separations vary less for HPLC than GC. High pressure is used in HPLC. Length of the column is small. GC is used for only volatile compounds mainly and only 15% of organic compounds are volatile but in HPLC both volatile and non volatile compounds are analyzed.
What is deference GC, HPLC & GCHS
I do not know what either of those things are but I bet there is a difference just letting you know that I think that HPLC stand for HEY.PLEASE.LICK.COCK. And GC stands for GO.CUNK
In HPLC, the carrier is liquid while in GC, the carrier is gas.
i have no answer for it...think yurself...
Whether GC or HPLC is a more preferable analytical method depends on the material being analyzed for, the likely concentration in which it is found, and what medium it may be in/on (water, oil, different kinds of filters, etc.) There is no single, universally applicable answer.
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
HPLC can separate nonvolatile species and GC cannot.
why RT was shifting & how to RT calculation in HPLC
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
HPLC purity :It explains how pure our analyte is in the given mixture .It is not related to the how much our analyte is in the given mixture.i.e Percentage of a our analyte with out impuritys in HPLC.(Known or Unknown)HPLC assay :It explains how much is our analyte in the given mixture(The content of our component in the given mixture).It is not related to analyte purity.HPLC potency :It is measurement of our analyte how potent it is.i.e Purity of our analyte with out all possible impuritys like chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy metals,sulphated ash ..etcFor example:If we have a analyte of some X of purity 99.5%.Prepare 20%,60% and 90% of solution of X.inject all these solution in hplc.For 20% solution you will get 99.5% purity and 20% assay.For 60% solution you will get 99.5% purity and 60% assayFor 90% solution you will get 99.5% purity and 90% assay.
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
The time required to elute unretained peak from column is known as void time
"RS-HPLC method" means "Related Substance HPLC Method".
CPT Code 82542- Column chromatography/mass spectrometry (eg, GC/MS, or HPLC/MS), analyte not elsewhere specified; quantitative, single stationary and mobile phase.
what is endcapping of hplc column
Jeep does not use inertia/reset switches.
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
done msc biotech and worked on hplc and gc. doing now reaserch on rice to know dominating genes by markera. how this vl help me getting a job
how long time gradient reach column.