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What is an elution buffer?

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2011-09-13 14:02:22
2011-09-13 14:02:22

In column chromatography, it is put in the column to basically cleanse and lubricate. Generally, it helps to wash out any left-over proteins from a previous experiment. It can also help to separate the fractions that are collected.

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Tris is used as a buffering agent in the elution buffer.

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In an elution buffer at room temperature.

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You need to consider the pH of the elution buffer of the mobile phase in a chromatographic run. You should work within 1 pH unit of the buffer pKa value.

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One consideration to be made when choosing an elution buffer is to make sure the pH is within the range of the desires. Stabilizing components will help increase the solubility and stability of your solution.


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