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  • Human Anatomy and Physiology

What is enzyme purity?

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βˆ™ 2011-03-03 15:55:51

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Can gel electrophoresis be used to determine the purity of an enzyme?

Yes it can be done if you use Poly-Acrylamide Gel Electrophoresis.


How long would it take 1 picomole of enzyme to convert 1 micromole of the substrate to product if the turnover number was 1 per millisecond?

Depends on enzyme purity, activity and incubation conditions.


What is a molecular weight of pectinase?

it is depend on the purity of pectinase enzyme. to know exact value of mw you have to run SDS page for individual sample.


Why is succinate dehydrogenase a good marker enzyme?

Isolation of any organelle requires a reliable test for the presence of the organelle. Typically, this is done by following the activity of an enzyme that is known to be localized exclusively in the target organelle. Such enzymes are known as marker enzymes. For example, the enzyme acid phosphatase (that cleaves terminal phosphate group from substrates and has a pH optimum in the acidic range) is localized in lysosomes, while the enzyme succinate dehydrogenase is localized in mitochondria. By monitoring where each enzyme activity is found during a cell fractionationprotocol, one can monitor the fractionation of lysosomes and mitochondria, respectively. Marker enzymes also provide information on the biochemical purity of the fractionated organelles. The presence of unwanted marker enzyme activity in the preparation indicates the level of contamination by other organelles, while the degree of enrichment for the desired organelle is determined by the specific activity of the target marker enzyme. Although marker enzymes reveal much concerning the purity of the organelle preparation, electron microscopy is generally used as a final step to assess the preparation's purity and the morphology of the isolated organelle.


Where can you get cheap purity rings?

Purity rings are a symbolism of the purity and abstinence in your life. You can purchase any ring from anywhere and it will symbolize your purity if you choose.


What is the technical term for purity of water?

Purity.


What do the white stripe represent?

purity purity


What were the purity crusaders?

people who crusaded purity


What are the factors that affect yield and purity?

purity


Reinheitsgebot is German for purity law but it's not racial purity What kind of purity does it govern?

Beer


How do you uses the word purity in a sentence?

Her purity made her a great inspiration. The purity of that food is in question.


How many ounces of gold are in a gold chain weighing 2.5 ounces?

The answer will depend on the purity of the gold.The answer will depend on the purity of the gold.The answer will depend on the purity of the gold.The answer will depend on the purity of the gold.


What is important about product purity?

There are many reasons why product purity is considered to be important. In regard to chemicals, the purity might change the effectiveness of the contents with a change in purity.


What is a purity necklace?

A Purity necklace is the same as a purity ring. The difference is it is just a necklace instead of a ring but it means the same thing. I have a purity necklace myself and if you are thinking about getting one it is a awesome thing to have. So like you can have a purity ring, purity necklace, purity bracelet any jewelry really it all means the same thing.


Greek word for purity?

Purity in Greek is 'hagnea.' :)


How do you check the hydrogen gas purity?

hydrogen purity


How do most religions address the question of purity?

Have purity of mind and especially purity of actions. Don't harm people.


What does karats mean is gold?

It is used to measure the purity of gold alloys. One karat (or carat) is equal to 1/24 purity by mass.24 carat (millesimal fineness 99.9% or higher)purity22 carat (millesimal fineness 91.6%)purity21 carat (millesimal fineness 87.5%)purity20 carat (millesimal fineness 83.3%)purity18 carat (millesimal fineness 75.0%)purity15 carat (millesimal fineness 62.5%)purity14 carat (millesimal fineness 58.5%)purity10 carat (millesimal fineness 41.7%)purity9 carat (millesimal fineness 37.5%)purity8 carat (millesimal fineness 33.3%)purity1 carat (millesimal fineness 04.2%)purity


How do you write purity in Hebrew?

The word Purity in Hebrew is "Tohara"


How do you measure purity of gold?

The purity of gold is expressed in karats,


Is Purity the Hedgehog a real character?

No, Purity is not an official character.


How we do purity of a substance?

Purity of a substance is evaluated by chemical analysis.


When was The Castle of Purity created?

The Castle of Purity was created in 1972.


What is the Irish for 'purity'?

glaineacht; íonacht; geanmnaíocht; geanas


What is the population of Purity Dairies?

The population of Purity Dairies is 500.