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A measure of how much more pure your protein is after a purification step in comparison to the crude. You can calculate this by dividing the purified steps specific activity by the crude steps specific activity.
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∙ 12y ago
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What does protein expression and purification mean?
Protein expression means to clone compounds. Purification is the cleaning process of those compounds. http://en.wikipedia.org/wiki/Protein
Is protein expression and purification a scientific process?
Yes, you are correct. Protein expression and purification is definitely a good example of scientific process.
Does total activity increase or decrease during protein purification?
Total Activity almost always decreases during protein purification.
Why you are adding gst tag to the protein during protein purification?
You have many proteins in a mixture. To identify a single protein you add a GST tag. So, when you are doing any further purification you will end up with your protein of choice.
What has the author Miguel Benitez written?
Miguel Benitez has written: 'Protein purification' -- subject(s): Proteins, Purification
What has the author Robert K Scopes written?
Robert K. Scopes has written: 'Protein purification' -- subject(s): Enzymes, Isolation & purification, Proteins, Purification
How protein fold and unfold?
Hhhvcdcfxf
What causes a protein to fold?
calcium causes that
What are the benefits of protein expression and purification?
Protein expression and purification is when proteins are taken and isolated for their individual benefits and then used in many products such as enzymes and in creams. The benefits are that the products they are used in are more natural and have a better balance with the body.
What is tertiary structure in protein?
The 3D shape or fold.
The PI of protein is 9.24 At what PH MY protein binds to the column and which type of column have to use for purifification?
The PI of protein is 9.24 At what PH MY protein binds to the column and which type of column have to use for purification?
How does the level of protein affect cystic fibrosis?
Fold improperly
