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to allow the excess water to dry out before heating. if heated right after, the water would cause the smear to overheat and denature some features in the stain. and those features would no longer be seen
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∙ 14y ago
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∙ 15y agoHeat will cause the capsule to shrink, making it look like there isn't one. Conversely, heat may cause a bacterial cell to shrink, making a clear zone around the cell, effectively making a cell appear to have a capsule when it doesn't.
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∙ 13y agoHeat fixation is performed by the rapid passage of the air-dried smear two or three times over the flame of the Bunsen burner.
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∙ 13y agoHeat_fixationis used to kill, adhere, and alter the specimen so it will accept stains
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∙ 12y agoThere is no heat fixing or water rinsing in capsule staining because they can destroy the capsule or give false positive results.
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∙ 11y agoWithout heat fixing it when you stain the slide and then wash it off to see the results that bacteria sample will wash it off with it. Also the heat fixes the bacteria in place.
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∙ 16y agoTo kill the smeared organism as well as to adhere the smear to the slide... go to the website link and scroll down until you find 'heat fixing.'
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∙ 12y agoyou just dont
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∙ 12y agoBacterial* Suspension*
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Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
What will happen if too much heat is applied while heat fixing a slide?
When too much heat is applied during the heat fixing of a slide with a bacterial cell on it, the cell would explode. The membrane of the cell would rupture.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
Why must smear on the slide be completely dry before heat fixation?
Slight heating helps in fixing the cells on to the surface of the glass slide
Can you heat fix blood smear before staining for bacterial examination?
You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
What effect does heat fixing have on bacterial cell size?
it makes cell size shrink
What effect does heat-fixing have on bacterial cell size?
it makes cell size shrink
What is the purpose of fixing the smear?
Heat fixing a smear kills the bacteria with minimal distortion, allows for better staining, and firmly affixes the bacteria to the slide. Chemical fixing is used to preserve fine cellular structures and might stop internal processes in place, protect the cell from damage, or strengthen the cell's structure.
What will happen if too much heat is applied while heat fixing a slide?
When too much heat is applied during the heat fixing of a slide with a bacterial cell on it, the cell would explode. The membrane of the cell would rupture.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
Why must smear on the slide be completely dry before heat fixation?
Slight heating helps in fixing the cells on to the surface of the glass slide
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
