What is role of buffer solution in complexometric titration?
buffer solutions resists change in PH
The role that tris-HCI plays in plasmid isolation is to maintain the pH of the solution. This prevents degradation of the plasmids. Tris stands for the organic compound, tris(hydroxymethyl)aminomethane, which is a common pH buffer. HCl is a salt acid called hydrochloride. This is added as a buffer as well to add stabilization.
TAE buffer is a buffer solution containing a mixture of acetic acid and EDTA. In molecular biology it is used in agarose electrophoresis typically for the separation of nucleic acids such as DNA and RNA. It is made up of tris-acetate buffer, usually at pH 8.0, and EDTA, which sequesters divalent cations. TAE has a lower buffer capacity than TBE buffer and can easily become exhausted, but linear, double stranded DNA runs faster in TAE…