What is shake flask fermentation?
Shake Flask fermentation is a cheapest ans simplest technique to
grow bacteria or fungi, aerobically, in small volumes of nutrient
broth. The broth is poured into erlenmeyer flasks equipped with
cotton-wool stoppers, and autoclaved. After cooling, some microbes
are "seeded" into the flask, and it is placed on a Shaker machine.
The shaking agitates the content and so ensures aeration, so that
the microbes could breathe.