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What is the difference between a simple and a differential stain?
Klsr2fine2
THE DIFFERENTIAL STAIN, SUCH AS THE GRAM STAIN, STAINS THE CELL WALL OF GRAM POSITIVE BACTERIA, DUE TO THE HIGH LEVEL OF PEPTIDOGLYCAN PRESENT IN THE CELL WALL, THESE WILL BE PURPLE IN COLOR. THOSE THAT STAIN GRAM NEGATIVE, DO NOT HAVE AS MUCH PEPTIDOGLYCAN IN THEIR CELL WALL AND WILL RETAIN NEGLIBLE AMOUNTS OF THE CRYSTAL VIOLET AND MORE OF THE SAFFRIN. HENCE THOSE WILL BE GRAM NEGATIVE OR RED TO PINK. THUS THIS TYPE OF STAIN DIFFERENTIATES THE BACTERIA IN TO TWO BASIC CATEGORIES. THIS ASSISTS THE PHYSICIAN IN MAKING A CHOICE REGARDING ANTIBIOTICS TO TREAT THE PATHOGEN WITH. A SIMPLE STAIN JUST SHOWS THE BASIC MORPHOLOGY OF THE BACTERIUM OR BACTERIA PRESENT. YOU MAY WISH TO USE BROMTHYMOL GREEN, CRYSTAL VIOLET... ETC. ALL YOU ARE LOOKING AT IS MORPHOLOGY. HOPE THAT HELPS
Wiki User
∙ 15y ago
Wiki User
∙ 9y agoA simple stain depends on the fact that bacteria differ chemically from their surroundings and thus can be stained with their environments.
A differential stain can distinguish betweeen 2 types of bacteria because bacteria differ from one another chemically & physically to react differently to a given staining procedure.
Wiki User
∙ 13y agoSimple stain , is just used to bring the bacteria from the fore ground so you can see it better, and it's heat fix so it's no longer alive.
Differential stain, is also heat fix, but you used Crystal violet which binds to the cell wall of the bacteria that have a thick cell wall, and Iodine is used as a mordant too help crystal violet attach to the cell wall, the counter stain is Safranin which then bind too bacteria that have less of a Cell wall, which makes them red/organ and the crystal violet make those bacteria purple. Hence you can differentiate between a Gram Negative which are the red/orange bacteria and the Gram Positive which are the Purple bacteria.
Wiki User
∙ 13y agoSimple staining allows you to view cellular structure. Differential staining allows you to identify the components that make up the cell wall (which helps to lead to genus and species identification of the organism).
Wiki User
∙ 14y agoUsing multiple stains can better differentiate between different microorganisms or components of a single organism.
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Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
What is the difference between Wright stain and Giemsa stain?
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
What is the advantage over the simple stain?
Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.
What is differential stain?
Using two different dye
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the pH is?
8
Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
Can you tell an organisms gram stain by simple staining?
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
What is the difference between Wright stain and Giemsa stain?
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
What is the advantage over the simple stain?
Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.
What is differential stain?
Using two different dye
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the pH is?
8
Difference between dye and stain?
A stain is temporary whereas dyes are permanent and can be removedonly after cell wall destruction.
A structural stain is considered to be a differential stain?
yup..two types of differential staining.. 1 - staining the whole cell to differentiate from another 2 - structural stainging of an individual cell
What is the difference between a varnish and a stain?
A stain is used to color the wood while a varnish is a topcoat used to protect the wood.
What is the difference between laundry detergent and stain removers?
Laundry Detergent Gets Rid Of Smells And Odors And Stain Removers Get Stains Out.
Why is a stain called a stain?
It's simple, because it's a stain.
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
