Leishman staining is used for staining blood in microscopy and its purpose is to both identify and differentiate trypanosomas, leucocytes and malaria parasites. Giesma staining is used to stain DNA region, specifically chromosomes in order to locate aberrations like rearrangement and translocations.
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
It's used to differentiate the different constituent of the blood cell
giemsa stains the phosphate group especially in the adenine-thymine rich region.
* Cells relatively large * Cytoplasm well displayed even in lymphocyte, oat cells,…etc. * Good for showing basophilia, cytoplasmic granules, and surface microvilia * Signs of degeneration easily visible * If mounted without delay, dyes fast for several decades. * Heterochromatin not shown in form familiar to histopathologist. * Hyperchromasia not recognizable * Nucleoli pale * Free cells may appear adherent * Three-dimensional structures not transparent * Slides useless if cells not concentrated enough to dry rapidly
they derive from a common ancestor. a homology is a "structural correspondence".Homology among proteins and DNA is often concluded on the basis of sequence similarity, especially in bioinformatics. For example, in general, if two or more genes have highly similar DNA sequences, it is likely that they are homologous. But sequence similarity may also arise without common ancestry: short sequences may be similar by chance, and sequences may be similar because both were selected to bind to a particular protein, such as a transcription factor. Such sequences are similar but not homologous.
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
Romanowsky staining was a prototypical staining technique that was the forerunner of several distinct but similar methods, including Giemsa, Jenner, Wright, Field, and Leishman stains, which are used to differentiate cells in pathologic specimens. A group of eosin-methylene blue stains generally used for blood smears, protozoa and bacteria.
It depends on what tissue you're looking at, what you want to stain, how the tissue has been stored... Besides very specific staining, there are different types of staining. For example, immunohistochemistry, which uses antibodies to stick coloured stains to cell surface receptors. Or, chemical staining - the most common is H&E staining (haemotoxylin & eosin), so if you're just having fun in a lab and want to see general structures of cells, use this one.
because giemsa stain is a mixture of methyl acetate Eosin and azure b. it doesnot contain any fixative that is why we use methanol to fix smear during giemsa stain other stain like lieshman contain acetyl free methyl alcohol as a fixative so it does not need to fix slide stain with lieshman stain.
Gustav Giemsa was born in 1867.
Gustav Giemsa died in 1948.
Edmund Giemsa was born in 1912.
Edmund Giemsa died in 1994.
The doctor will look for the presence of follicles or scarring. He or she will take a small sample of cells from the patient's conjunctivae and examine them, following a procedure called Giemsa staining, to confirm the diagnosis.
It's used to differentiate the different constituent of the blood cell
giemsa stains the phosphate group especially in the adenine-thymine rich region.
Giemsa stain binds the regions with high content of adenine-thymine complex.