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It allows the biologists to make many copies of a dna strand...
Wiki User
∙ 12y ago
Wiki User
∙ 12y agoTo denature the double stranded DNA. ie Danaturation
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What is the first step of a polymerase chain reaction?
Denaturation of double strand(above 90degree C)
In polymerase chain reaction technology the two strands of DNA are separated by?
In polymerase chain reaction everything is done by temperature with the help of some enzyme. In first step the templet strand is separated by high degree of temperature because in higher temperature the hydrogen bonds between the A-T and G-C are digested.
What is the PCR technique?
PCR (Polymerase Chain Reaction) is used to amplify a small amount of genetic material into a much larger amount in a relatively short amount of time.PCR (Polymerase Chain Reaction) is used to amplify a small amount of genetic material into a much larger amount in a relatively short amount of time.
Could you use human polymerase for the extension step in the PCR reaction?
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
Scientist wants to insert a new gene into a bacterium. What is the first step in this process?
Cutting the gene out of DNA with enzymes - gradpoint
What is the first step of a polymerase chain reaction?
Denaturation of double strand(above 90degree C)
What the second step in the Polymerase chain reaction process?
anealing
If e. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure what would happen?
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
In polymerase chain reaction technology the two strands of DNA are separated by?
In polymerase chain reaction everything is done by temperature with the help of some enzyme. In first step the templet strand is separated by high degree of temperature because in higher temperature the hydrogen bonds between the A-T and G-C are digested.
If E. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure then will reaction be carried out in waterbath?
no e coli DNA pol can not with stand high temperature if so for every denaturation step we must add fresh enzyme
A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?
Run gel electrophoresis :: Apex
What is the PCR technique?
PCR (Polymerase Chain Reaction) is used to amplify a small amount of genetic material into a much larger amount in a relatively short amount of time.PCR (Polymerase Chain Reaction) is used to amplify a small amount of genetic material into a much larger amount in a relatively short amount of time.
Could you use human polymerase for the extension step in the PCR reaction?
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
Which molecule is active during the last step during DNA replication?
DNA ligase. Apex
What are the differences between additional polymerization and condensation polymerization?
the structural unit of step reaction polymerization are chemically identical to the monomer employed in the reaction while those of the chain reaction may or may not be identical addition or step polymers take their names from their starting monomers while those of the chain reaction is not so
What is the first step of the chain of civilization?
agriculture.
What is chain termination?
Chain termination is any chemical reaction leading to the destruction of a reactive intermediate in a chain propagation step in the course of a polymerization, effectively bringing it to a halt.
