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What is the function in the following reagents used in the extraction and precipitation of DNA?
Wiki User
∙ 11y ago
to extract the DNA from the solution, isopropyl alcohol is mixed with the DNA solution to precipitate the same.
Wiki User
∙ 11y ago
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What is the point of mashing straberrys for DNA extraction?
Use the usual extraction protocol for plants: grind, liquid-liquid extraction, salting out.
Electrophilic and Nucleophilic Reagents?
ELECTROFILIC REAGENTS:It is the combination of two words(electro means electron and phile means loving).Therefore those reagents that contain positive charge on it are called eletrophilic reagents. These reagents have the property of attacking on the negative part of the other substance.Some of the reagents are(NO2+ ,SO3+) NUCLEOPHILIC REAGENTS:Combination(nucleo means nucleus and phuile means loving)
Why do you keep reagents on ice?
Reagents are kept on ice to prevent denaturation or any form of unwanted reactions.
What is different between electrophilic and nucleophilic substation?
Electrophilic reagents are chemical species which in the course of chemical reactions, acquire electrons or a share in electrons from other molecules or ions. Nucleophilic reagents do the opposite of electrophilic reagents.
What are salts that separate ions refered to as?
These are precipitating reagents.
Why saturated KCL in DNA extraction is used?
Saturated KCl precipitation is often used in DNA extraction for molluscan taxa. Molluscs produce a polysaccharide rich mucus that interferes with the reagents involved in DNA extraction. The KCl saturated solution is used right after the digestion step: about 1/4th of the volume of the digestion solution is added to the sample. Samples are then centrifugated at 14rpm for 15 minutes. The pellet formed will contain the polysaccharides and non digested tissue. The supernatant is extracted from the tube and used in the next steps of the DNA extraction.
What is the function of a wide mouth bottle?
This bottle is used to contain reagents.
Difference between precipitation titration and complexometric titration?
During a precipitation titration an insoluble precipitate is formed.Complexometric titration is a type of volumetry which use as titrants EDTA or other similar reagents.
What is the point of mashing straberrys for DNA extraction?
Use the usual extraction protocol for plants: grind, liquid-liquid extraction, salting out.
Why is it necessary to test for complete precipitation if a supernatant solution is going to be subsequently tested for ions?
The presence of unprecipitated ions in the supernatant may react with subsequent reagents added and obscure the results of tests.
What is the purpose of water washing the organic layer?
The purpose of water washing the organic layer is for aqueous extraction to remove impurities and or reagents that are water soluble. For instance, in reactions that utilize a small tertiary amine base or inorganic base.
What do you mean by reagents in chemistry?
Chemical compounds used in laboratory are frequently called reagents.
Can chemicals and reagents go bad?
Yes only some... It depends
What are the reagents used in the estimation of antioxidants?
There are various reagents that are used in the estimation of antioxidants. Folin-Ciocalteu and DPPH are the most common reagents used in determining antioxidant activity.?æ
Electrophilic and Nucleophilic Reagents?
ELECTROFILIC REAGENTS:It is the combination of two words(electro means electron and phile means loving).Therefore those reagents that contain positive charge on it are called eletrophilic reagents. These reagents have the property of attacking on the negative part of the other substance.Some of the reagents are(NO2+ ,SO3+) NUCLEOPHILIC REAGENTS:Combination(nucleo means nucleus and phuile means loving)
Why are Grignard reagents synthesized in an ether medium?
Grignard reagents are stable in ether medium. Ether generally forms an adduct with Grignard reagents. In addition, if solvents containing acidic hydrogens are present, Grignard reagents are decomposed to the corresponding hydrocarbon.
Why do you keep reagents on ice?
Reagents are kept on ice to prevent denaturation or any form of unwanted reactions.
