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to resist drastic changes in the pH of a solution
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∙ 14y ago
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the function of a buffer is to maintain the pH of the sample.
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We use it for isolation of proteins from yeast cells as a lysis buffer
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Why gets function gives a warning every time you compile a c program?
It is unsafe. In order to use gets() safely, you need to know how many characters you will be reading to ensure your character buffer is large enough: char buffer[10]; while (gets (buffer) != 0) { ...process buffer... } The above code has undefined behaviour when the number of characters read is 10 or more (you need one character for the null-terminator). This is because the character buffer, str, decays to a pointer (referencing &str[0]) and the function, gets(), cannot determine the number of characters in a buffer by its pointer alone. The gets() function was dropped from the C standard in 2011, however some implementations still include it. To avoid the warning, use the fgets() function instead. This allows you to specify the length of your buffer and (when used correctly) prevents buffer overflow. char buffer[10]; while (fgets (buffer, 10, stdin) != 0) { ...process buffer... }
What is the role of mgcl2 in the lysis buffer?
MgCl2 is added to the lysis buffer since Mg2+ ions are co-factors for the enzyme used in the lysis buffer. This enzyme requires magnesium ions in order to function properly.
Function of binding buffer?
When alkali or acid is added to a pH solution, a binding buffer will help prevent the pH from changing. There is also the elution buffer which is used to clean out any proteins which are leftover.
What is the difference between tbs buffer-tbst buffer and corresponding function of them?
Tween 20. In TBST you add 0.05-0.1/ Tween 20.
