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ammonium acetae use to percipitate DNA from water.
how to make sodium citrate in 10% ethanol for DNA extraction
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In lysis buffer, the function of ammonium chloride is to determine the red blood cells in samples. These samples also contain white blood cells.
To achieve precipitation DNA.
chelating Mg2+
DTT is the reducing agent for thiolated DNA
It solubalize lipids and protiens to remove them from DNA
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
removal of polyphenols
to remove excess phenol from DNA to remove excess phenol from DNA
Triton X-100 is used as a lysis buffer for DNA separation.