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Function of Gram's iodine

Updated: 8/11/2023
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13y ago

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Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.

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9y ago
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12y ago

Iodine serves as a mordant by binding to the crystal violet. An insoluble iodine-crystal violet complex is formed that allows gram positive bacteria to remain purple after this step in the gram staining procedure.

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13y ago

Iodine act as modrant for crystal violet and helps its attachement more firmly.

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Q: Function of Gram's iodine
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