positive result for when buiret is added to protein is blue or violet/pale lilac colour. (not pink).
positive result for when iodine is added to starch is blue or black colour (not pink).
The biuret test is a chemical test used for detecting the presence of peptide bonds. In a positive test, a copper(II) ion is reduced to copper(I), which forms a complex with the nitrogens of the peptide bonds in an alkaline solution. A violet color indicates the presence of proteins.
Copy and pasted straight from wikipedia. Did you do any research at all before asking this? do your own homework.
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just sayin, wikipedia is unreliable, but yea in this case its right. the solution will turn pink/violet.
The Biuret reagent is made of potassium hydroxide (KOH) and hydrated copper(II) sulfate, together with potassium sodium tartrate and has blue colour.
The reagent turns from blue to violet in the presence of proteins, blue to pink when combined with short-chain polypeptides.
It will change violetish because the solution reacts with the peptide bonds that link the individual amino acids into the protein chains.
A Biuret test gives positive when it turns violet or pink.
When tested with iodine, you will see a blue-black colour if starch is present.
If there is no starch present, the iodine will remain brown.
In the Biuret test, the blue reagent turns violet it it detects proteins, and changes to pink when combined with short-chain polypeptides.
deep blue/purple color
Proteins
purple
No. The Biuret test only gives a positive result if a peptide bond exists. Phenol doesn't have a peptide bond, so it will not give a positive test.
The conclusion is that the sample contain proteins.
No because glucose does not consist of two or more peptide bonds
no! no change in colour confirms presence of cysteine
Hey. okay.. the biuret test is a test for peptide bonds. when CuSO4 is added to KOH, an alkaline solution, nitrogen atoms from the peptide bond forms a purple compound with the Cu2+ ions. hope this helps! read it in a text book.
you should have at least 2 of these,to obtain a positive result in Biuret's test. because biuret test is a test used to detect if there is peptide linkages.
No. The Biuret test only gives a positive result if a peptide bond exists. Phenol doesn't have a peptide bond, so it will not give a positive test.
The biuret solution used in the biuret test for peptide bonds is blue in the absence of peptide bonds or biuret which also results in a positive result. A positive result for peptide bonds or biuret is apparent as the biuret solution turns purple/violet in color.
Yes, the biuret test will indicate the presence of peptides. If it turns violet, it is a positive result.
The conclusion is that the sample contain proteins.
No
If Biuret Reagent is related to the presence of Protein macromolecules, then a positive result will change it's colour from blue to violet.
No because glucose does not consist of two or more peptide bonds
Amino acids doesn't react with the biuret reagent.
A positive test, when using Biuret reagent, would be purple-ish
Yes. The Biuret test is a test for the presence of peptide bonds. Since albumin is a class of protein, and proteins are composed of many amino acids joined together by peptide bonds, the test will yield a positive result.
no! no change in colour confirms presence of cysteine