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Gene splicing means cutting of gene in fragments and rejoining them according to need There are two phenomenon by which gene splicing occur one is natural i.e. post transcriptional modification and other is artifically or by chemical agent used generally in recombinant technology

POST TRANSCRIPTIONAL MODIFICATION: Gene splicing is a post-transcriptional modification in which a single gene can code for multiple proteins. Gene Splicing is done in eukaryotes, prior to mRNA translation, by the differential inclusion or exclusion of regions of pre-mRNA. Gene splicing is an important source of protein diversity. During a typical gene splicing event, the pre-mRNA transcribed from one gene can lead to different mature mRNA molecules that generate multiple functional proteins. Thus, gene splicing enables a single gene to increase its coding capacity, allowing the synthesis of protein isoforms that are structurally and functionally distinct. Gene splicing is observed in high proportion of genes. In human cells, about 40-60% of the genes are known to exhibit alternative splicing.

Gene Splicing Mechanism

There are several types of common gene splicing events. These are the events that can simultaneously occur in the genes after the mRNA is formed from the transcription step of the central dogma of molecular Biology.

Exon Skipping: This is the most common known gene splicing mechanism in which exon(s) are included or excluded from the final gene transcript leading to extended or shortened mRNA variants. The exons are the coding regions of a gene and are responsible for producing proteins that are utilized in various cell types for a number of functions.

Intron Retention: An event in which an intron is retained in the final transcript. In humans 2-5 % of the genes have been reported to retain introns. The gene splicing mechanism retains the non-coding (junk) portions of the gene and leads to a demornity in the protein structure and functionality.

Alternative 3' splice site and 5' splice site: Alternative gene splicing includes joining of different 5' and 3' splice site. In this kind of gene splicing, two or more alternative 5' splice site compete for joining to two or more alternate 3' splice site.

A gene-sized fragment of DNA is isolated from another organism. The bases of the fragment are spliced (joined) to the bases of the molecule by using a chemical called ligase.

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โˆ™ 2009-10-16 20:06:26
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Q: What is the process of a gene splicing?
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What is one benefit of gene splicing?

Gene splicing is the process of taking fragments from DNA . One benefit of gene splicing could be to alter the health of an ill person by using the splicing technique to manipulate the unhealthy genes.


Thought Out what process gene from one organism can be combined with a gene from another organism?

Splicing


What can be accomplished by splicing genes?

Alternative splicing is a process that occurs during gene expression. It allows for the production of multiple proteins from a single gene coding.


Alternating RNA splicing?

During gene expression a regulated process is alternating RNA splicing. It has a single gene coding that is used for many proteins.


What is the process of coping an RNA message from the DNA code?

gene splicing


What process is used to get the foreign gene into the new organism?

You would need to use Gene splicing to insert a foreign gene into an organism.


What is an alternative RNA splicing?

An alternative RNA splicing is a process by which the exons of the RNA produced by the transcription of a gene are reconnected in multiple ways during RNA splicing, so as to allow production of multiple forms of protein from one gene.


What is the method of gene-splicing?

Gene splicing is the method of cutting out a part of the DNA in a gene and adding a new DNA in it's place.


Who started gene splicing?

Spicies


Why does gene splicing work?

Gene splicing is one of the important step in central dogma of eukaryotic cells Gene splicing is a post-transcriptional modification in which a single gene can code for multiple proteins. Gene Splicing is done in eukaryotes, prior to mRNA translation, by the differential inclusion or exclusion of regions of pre-mRNA. Gene splicing is an important source of protein diversity. During a typical gene splicing event, the pre-mRNA transcribed from one gene can lead to different mature mRNA molecules that generate multiple functional proteins. Thus, gene splicing enables a single gene to increase its coding capacity, allowing the synthesis of protein isoforms that are structurally and functionally distinct. Gene splicing is observed in high proportion of genes. In human cells, about 40-60% of the genes are known to exhibit alternative splicing.


What is gene splicing used to produce?

Insulin


What is the definition of the word intron?

The definition of the 'intron' is part of the DNA sequence within a gene. It is the sequence within a particular gene that is removed during the RNA splicing process.

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