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What is the purpose of the ampicillin in the transformation procedure?
Wiki User
∙ 12y ago
The ampicillin resistance gene (AmpR) is called a selectable marker gene and is incorporated into several plasmids that are commonly used in a wide range of genetic engineering and molecular Biology experiments.
The function of a selectable marker gene is to provide the host containing the plasmid a certain property that is not inherently present in the host. For example, the AmpR gene codes for a protein that makes cells containing this gene resistant to the antibiotic ampicillin.
Therefore, when plasmids are successfully transfected into bacterilal cultures, one can assess which colonies have taken up the plasmid by looking at which ones survive onampicillin-containing media. Those that do not survive do not have the plasmid. The surviving colonies can then be picked up and carried on to the next stage of experimental research.
Wiki User
∙ 15y ago
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The plasmid pARA-R has been genetically modified for what purpose?
ampicillin resistant
How ampicillin synthesised?
how ampicillin and amoxycillin synthesised
Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?
The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.
Why do the cells transformed with pUC18 and plasmid lux grow in the presence of ampicillin?
they both contain an ampicillin resistent gene
Why is ampicillin added to the pound medium?
Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means no plasmid in the bacterial host...
The plasmid pARA-R has been genetically modified for what purpose?
ampicillin resistant
Why is ampicillin important?
ampicillin is a antibiotic
How ampicillin synthesised?
how ampicillin and amoxycillin synthesised
Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?
The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.
How do ampicillin resistant E. coli combat the lethal effects of the ampicillin antibiotic?
The pGlo plasmid contains an ampicillin resistance gene. This gene encodes an enzyme, β lactimase, which enzymatically degrades ampicillin. Therefore, bacteria that take up the plasmid (transformants) become resistant to ampicillin.
How long does ampicillin stay in your system?
Half life of ampicillin is 1 hour.
What is the purpose of the procedure?
The purpose of Parliamentary Procedure is to keep a meeting running smoothly and to make sure everyone has a fair say in what is proposed to do.
Does ampicillin affect the contraceptive implant?
There are no known drug interactions between ampicillin and the contraceptive implant.
Can you use alcohol when taking ampicillin tr 500mg?
there are no known interactions between alcoholand Ampicillin
How does ampicillin work?
Ampicillin is in a class of medications called antibiotics. Ampicillin destroys the bacterial cells that cause many illnesses. Antibiotics are also notorious for killing the good and bad bacteria.
What is the classifications of ampicillin?
Ampicillin is an aminopenicillin that has antibiotic properties. Please see its page on wikipedia for more information.
Why do the cells transformed with pUC18 and plasmid lux grow in the presence of ampicillin?
they both contain an ampicillin resistent gene
