0
Cite the purpose of following reagents in a differential staining procedure?
Wiki User
∙ 9y ago
To distinguish between acid fast positive and acid fast negative bacteria. Acid fast positive bacteria will stain red to pink color and acid fast negative bacteria will stain blue. Acid fast positive bacteria have mycolic acid in their cell wall, which will stain with carbol fuchsin and not decolorize with acid alcohol. Acid fast negative bacteria do not have mycolic acid in their cell wall, and become decolorize with the acid alcohol. Counterstain of methylene blue needs to be done in order to see the acid fast negative.
Wiki User
∙ 11y ago
Wiki User
∙ 9y agoA differential stain can discern between two different types of bacteria. The names and purposes of the following reagents are: Mordant - A Gram iodine (named after Hans Christian Gram) stains positive bacteria crystal violet and the negative bacteria a red or pink color. Decolorizer - 95% percent ethanol is used to wash out crystal violet. Counterstain - a stain that will add a contrasting color to the principal stain which makes the stained structure easier to see.
Wiki User
∙ 15y agoto turn the non-acid cells a different color than the acid-fast cells
Wiki User
∙ 14y agoTo visualise what is not stained in the original stain.
Wiki User
∙ 9y agoIn differential staining procedure the purpose of counter stain is to give a color of red to a gram negative bacteria. Counterstaining dye used is usually safranin.
Wiki User
∙ 12y agoA stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the primary stain.
Add your answer:
Earn +20 pts
A structural stain is considered to be a differential stain?
yup..two types of differential staining.. 1 - staining the whole cell to differentiate from another 2 - structural stainging of an individual cell
Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
What is the different staining technique in virology?
1- What_is_the_different_staining_technique_in_virology2- What are the diffrent stain in micro for virus ?
Why gram staining is classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
What staining procedure will be used to observe bacteria in the sputum sample?
acid fast staining or Ziel Neelson staining for observing Mycobacteria tuberculosis or Koch bacilli from sputum sample.
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
What is the best staining procedure to view spirochaetes?
Fontana silver staining.
What are the disadvantages of negative staining in microbiology?
It sometimes require additional chemical reagents to produce the desired action.
A structural stain is considered to be a differential stain?
yup..two types of differential staining.. 1 - staining the whole cell to differentiate from another 2 - structural stainging of an individual cell
Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
What is the purpose of mordant in a differential staining procedure?
The resultant crystal violet- iodine (CV-1) complex serves to intensify the-color of the stain. At this point, all cells will appear purple black.
What is the purpose of mordant stain in a differential staining procedure?
The resultant crystal violet- iodine (CV-1) complex serves to intensify the-color of the stain. At this point, all cells will appear purple black.
What is the different staining technique in virology?
1- What_is_the_different_staining_technique_in_virology2- What are the diffrent stain in micro for virus ?
Why gram staining is classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Principle and procedure of histological staining of carbohydrates?
Carbohydrate demonstration methods. Demonstrates Staining mechanisms and technical comments.
