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I believe the role of proteinase K in a DNA isolation is just to digest proteins. Proteinase K is a protein digesting enzyme. Digesting proteins is important in a DNA isolation because the proteins included in your DNA before you treat it with proK likely include some DNAses. If you didn't use proK, your DNA would degrade very quickly.
Wiki User
∙ 15y ago
Kiko Garcia
It is worth noting that proteinase K is usually used in pre-hybridization processing in in situ hybridization technology. It has the effect of digesting and surrounding the target DNA protein to increase the chance of the probe binding to the target nucleic acid and improve the hybridization signal. But proteinase K's When the concentration is too high, the digestion time is too long, or the incubation temperature is too high, it will damage the structure of the cells to some extent, leading to the shedding of tissue sections and the disappearance of the nucleus, which will affect the results of hybridization. EDTA buffer can replace the role of proteinase K. Solve the above problems and achieve the ideal dyeing effect. You can learn more at the Creative Enzymes Website.
Wiki User
∙ 11y ago[proteinase k is a very good enzyme which degrade most of type proteins impurities in DNA isolation from blood so we can get good qualitative DNA from the blood
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What is the function of Proteinase K in DNA extraction?
Proteinase K is most often used in extracting DNA from while blood cells (or leukocytes). The first step in DNA isolation is to break open the cell and release the cytoplasmic contents, which includes the nucleus, in which we find DNA. Proteinase K is a protease (an enzyme capable of digesting proteins). It is used to digest the cell surface proteins. When cell surface proteins are digested, the integrity of the cell membrane is compromised leading to cell lysis (or the breaking open of the cell)
Where is double helix located?
In the DNA. The term "double helix" refers to the structure of DNA.
Which activity occur during the S of the cell cycle?
DNA COPYING , k .
What is the role of the promoter in transcription?
I have no f*ckin idea i was wonderign the same- george k
What are the two hormones you can't live without?
1. Cortisol - maintains glucos homeostasis 2. Aldosterone - plays a major role in Na+ and K+ levels in the body
What is the function of Proteinase K in DNA extraction?
Proteinase K is most often used in extracting DNA from while blood cells (or leukocytes). The first step in DNA isolation is to break open the cell and release the cytoplasmic contents, which includes the nucleus, in which we find DNA. Proteinase K is a protease (an enzyme capable of digesting proteins). It is used to digest the cell surface proteins. When cell surface proteins are digested, the integrity of the cell membrane is compromised leading to cell lysis (or the breaking open of the cell)
Where is double helix located?
In the DNA. The term "double helix" refers to the structure of DNA.
What has the author Robert K Scopes written?
Robert K. Scopes has written: 'Protein purification' -- subject(s): Enzymes, Isolation & purification, Proteins, Purification
What is the role of carbon in photosynthesis and respiration?
K
What is another name for the DNA material located within the nucleus of a cell?
chromatin
Which activity occur during the S of the cell cycle?
DNA COPYING , k .
What is colloidal system role in the cell cytoplasm?
k;
What role is played by a TNC subsidiary?
;kl;k;
An important role of vitamin K is the synthesis of?
prothrombin
What is the change of DNA when a guys eat another guys sperm?
What the actual f**k
What is the dramatic portrayal of a character called?
A Dramatic Role.
What has the author S K Ouki written?
S. K. Ouki has written: 'The Role of coagulants in water and wastewater treatment'
