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If you have a spectrofotometer ( the thing to mesure the absorbance)
then play with the setting and use a maximum.
this will lay close to your specific absorbance
or take the pharmacopea or a MERCK index
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∙ 11y ago
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What is the specific absorbance of hypericin?
Hypericin salts are red in organic solvents and show a typical absorbance at 590 nm, which is useful to quantify hypericin in the drug extracts
Why is absorbance plotted instead of percent transmission when following bacterial growth?
Because when we read absorbance, it's the amount of light absorbed by the bacteria itself. Absorbance is directly related to the amount of bacteria. More absorbance = more bacteria.
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
Is there a simple equation to calculate the concentration given the absorbance and a time?
You need a graphic concentration versus absorbance.
What are two reasons why absorbance measurements are taken at lambda max?
to ensure maximum absorbance of light by the solution
What is difference between specific absorbance and absorbance interms of spectroscopy?
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
What is the specific absorbance of hypericin?
Hypericin salts are red in organic solvents and show a typical absorbance at 590 nm, which is useful to quantify hypericin in the drug extracts
Is wavelength or absorbance the dependent variable?
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
What is the purpose of the blank?
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
What is the purpose of blank samples?
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
What is the different between primary absorbance and secondary absorbance in fluorescence?
in primary light absorbed by outer molecule while in secondary re-absorbance occurs
What is the relation of Beers Lambert's law in UV Visible spectrometer?
The Beer-Lambert law Absorbance = (extinction coefficent)(pathlength of light)(concentration) allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time. the pathlength of light being the width of the cuvette and the extinctin coefficent being specific to the product molecule.
Why is absorbance plotted instead of percent transmission when following bacterial growth?
Because when we read absorbance, it's the amount of light absorbed by the bacteria itself. Absorbance is directly related to the amount of bacteria. More absorbance = more bacteria.
What is the abbreviation for Absorbance?
A
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
Is there a simple equation to calculate the concentration given the absorbance and a time?
You need a graphic concentration versus absorbance.
What are two reasons why absorbance measurements are taken at lambda max?
to ensure maximum absorbance of light by the solution
