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There are several substances and mechanisms that cause muscle pain. The most common are immediate onset and delayed onset pain. Immediate onset pain is caused by muscle fatigue which comes from exercise of physical activity and occurs when the muscle has rerached its conditioned fatigue point. Delayed onset pain occurs 24-48 hours after the muscles have been worked to their fatigue point and are rebuilding themselves. This is the muscle soreness that many people experience when they start a new exercise program.

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Q: What is the substance that creates pain in human muscles?
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