What is used chromatography?

Answer 1

It is the separation of two or more substances in a solvent.

Chromatography is a separation technique based on the different interactions of the compounds with the two phases, a mobile phase and a stationary phase, as the compounds travel through the supporting medium. The mobile phase is the ink spot, as it travels up the piece of paper (in paper chromatography). The stationary phase is the piece of paper, or the different dyes when they stop travelling. There is also thin-layer chromatography, in which silicon is used instead of paper, which is basically the same concept. Chromatography is used to analyze, identify, quantify the compounds.

Please do note that the substances must have different solubilities in the solvent, and that they must have different colours. And for colourless liquids, tracking agents could be used to 'spot' or 'track' the liquid's progress up the piece of paper.

Different substances have different Rf values. The Rf value of a particular colouring is the distance travelled by the colouring over the distance travelled by the solvent.

Answer 2

Color writing. "Chroma" is a Greek roots prefix for color and "graphy" is a Greek roots suffix for writing. Answer:Chromatography is an analytical method to separate the compounds from the two immiscible phases,one being a mobile phase and another being a stationary phase.

Answer 3

Chromatography refers to a set of techniques used to separate different compounds. The word comes from the Greek chromatos (color) and graphein (to write). So, as one might guess, chromatography involves separating chemicals and identifying them by color.

Chromatography has many uses. It is commonly used in laboratories to isolate new compounds, analyze subtle differences between different environmental samples, and even in the sequencing of DNA.

To perform chromatography, one needs two things a matrix and a color indicator. A matrix is simply the material to which a sample is applied. the material is often porous, acting as a filter that affects the rate of flow of a sample. Special beads with different pore sizes are commonly used to separate proteins in biochemistry labs.

Answer 4

It is useful to separate and identify small amounts of substances that are dissolved in a solvent such as dyes in colored ink.

It can also be used to separate different components in a mixture based on the following principles:

-Different components in a mixture has different solubility in a solvent.

-The different solubility in a solvent causes them to move at different speeds on the chromatography paper