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What methods can be used to study protein-protein interactions?
Wiki User
∙ 14y ago
There are numerous methods for protein protein interactions. Biochemical methods include: 1) Coimmunoprecipitation - proteins are isolated with specific antobody. 2) Affinity Electrophoresis- estimates protein binding. 3) cross linking - to fix protein interactions before isolating 4) Quantitative immunoprecipitation: to detect interactions between endogenous non tagged proteins. Molecular Biology methods: 1) protein probing 2) two hybid system 3) phage display Genetic methods: 1) Isolation of extragenetic suppressors ,Synthetic mutants
Wiki User
∙ 14y ago
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