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Several factors affect the rate at which enzymatic reactions proceed - temperature, pH, enzyme concentration, substrate concentration, and the presence of any inhibitors or activator
Alvah Stokes
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What is the purpose of GUAIACOL?
Guaicol is used as a substrate for peroxidase activity, when we treat it with enzyme the activity of enzyme increases at a higher rate.
What affect does too much substrate have on the rate of an enzyme reaction?
The enzyme becomes saturated by the substrate and enzyme activity plateaus.
This essential substance increases the rate of a biochemical reaction?
the inorganic substance which increases the enzyme activity is called?
How do you calculate specific activity of salivary amylase?
Divide the rate of enzyme activity by the protein concentration.
What happens when an enzyme catalyzes a reaction?
The rate of a reactions usually increases when catalyzed by an enzyme. For maximum rate of activity, the enzyme needs optimal conditions.
What is the enzyam activity?
The enzyme activity depends on the conditions of the reaction and the enzmye used. The term enzyme activity refers to the rate at which substrate is converted into product and can be measured in many different ways.
Is there any Procedure in enzyme and factors that affect enzyme activity?
pH, temperature, substrate concentration and enzyme concentration influences the rate of reaction
What is the effect that lowering the temperature would have on the rate of enzyme activity explain?
It would lower the speed of the reaction but will do no harm to the enzyme.
What is the relationship between substrate concentration and enzyme activity?
At low substrate concentrations, the rate of enzyme activity is proportional to substrate concentration. The rate eventually reaches a maximum at high substrate concentrations as the active sites become saturated.
Why does increase enzymes concentration promote enzyme activity?
Increasing enzyme concentration increases the number of collisions between the enzyme molecules and the substrate molecules. This increases the number of successful collisions and the number of enzyme-substrate complexes. Therefore the reaction rate is increased as well and enzyme activity is promoted.
How does initial concentration of substrate effect enzyme activity?
At low concentration of substrate , rate of enzyme action is directly proportional to conc. of substrate .
What is the Effect of dilution on enzyme activity?
At a high ion concentration, the ion interfere with the bonds between the side groups of the amino acids making up the enzyme (which is a protein). This causes the enzyme to lose its shape, called denaturation. If the enzyme loses its shape, it can no longer accept and react substrate, so the rate of enzyme activity decreases.
