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Papain contains protease which is an enzyme that is responsible for removing the proteins in DNA. Which would be the histones, in which DNA is wrapped around

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Q: What role does papain play in the extraction of DNA?
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What is the function of CL buffer in DNA extraction?

In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.


Role of te in dna extraction?

TE stands for Tris and EDTA. The Tris buffers the water to prevent acid hydrolysis of the DNA/RNA. The EDTA chelates divalent cations that can assist in the degradation of RNA.


What is the principle of DNA isolation using phenol chloroform method?

Phenol chloroform extraction is the oldest and still widely followed method for the isolation and extraction of DNA from plant and animal cells. The phenol, chloroform (and also isoamyl alchohol) are added in a specific ratio of 25: 24:1.Phenol: Phenol dissolves the organic impurities, like proteins etc.chloroform: Provides density to phenol so that it settles below water during phase separation.Isoamylalchohol: Used to prevent phosgene from reaction of chloroform with air.The Phenol:Chloroform:Isoamylalchohol (PCI) solution is added to the cell extract after removal removal of debris. After proper mixing, cetrifugation is done to separate the phases. Two phases are formed: The upper, the aqueous phase that contains DNA, the lower phase, that phenol phase, that contains organic impurities. Thus two phases are separated by a very clearly defined boundary of coagulated proteins.The aqueous phase is precipitated and then the DNA could be pelleted after rounds of purifications.


What material carries the information that determines genetic traits?

DNA (Deoxyribonucleic acid)


What is DNA cloning?

DNA cloning is the production of large number of identical DNA molecules from a single ancestral DNA molecule. It is of two types 1. cell based DNA cloning 2. cell free DNA cloning