What s an anticoagulant?
Having the effect of retarding or inhibiting the coagulation of the blood.
First it should be clear that blood can't clot only in the presence of a anticoagulant or/and in the absence of clotting factors. if anticoagulant is not there in the vicinity of blood then blood will surely clot. Now it is clear that there is some anticoagulant in the blood vessels and that natural anticoagulant is named as heparin blood vessel coated with galyx
Does human body produce heparin and if our own body produces natural anticoagulant why do we need anticoagulant when we perform apheresis or dialysis?
Historically, EDTA has been recommended as the anticoagulant of choice for hematological testing because it allows the best preservation of cellular components and morphology of blood cells.The use of EDTA for measuring cytokines, protein and peptides, and cardiac markers is described, with an outline of the protection of labile molecules provided by this anticoagulant. The use of EDTA in proteomics and in general clinical chemistry is also described in comparison with other anticoagulants and with…
Usually, the vaccum in the tube is such that it cannot really be overfilled. Sometimes, blood is collected using a plain syringe and then put into a blue top. This can easily be the cause of cause overfilling. As for the effects, the ratio of the blood to anticoagulant would be such that there is less anticoagulant. This can cause the specimen to clot, and/or slightlydecrease the coagulation results. Less anticoagulant, clots faster.