Infectious Diseases
Microbiology
Bacteria

What would be the appearance of the gram positive bacterium if you forget to counterstain with safranin?

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October 21, 2012 12:12PM

If you forget to counter stain color of Gram positive would be violet or blue .

The above answer is good. Here is why the above answer is good.

Yes it would still be Violet or blue. Gram positive bacteria are gram positive, because it holds onto the crystal violet stain that washes out of gram negative bacteria. Counterstaining with safranian turns gram negative bacteria pink to red only because the crystal violet has washed out of the gram negative. The lighter safranian has little to no effect on gram positive bacteria.

The cause of the difference has to do with the makeup of the cell wall in the different bacteria.