If you mean what time of day, it doesn't matter. But it is said that you will have the highest level of the pregnancy hormone with your first pee of the day. If you mean what time of the month, then it is after you have missed your period. Some tests can be taken a few days before your period is supposed to start.
If you got your period, then most likely you are not pregnant. If you mean how soon after a missed period can you take a test then you can take a test the day that you were supposed to start. If you think that maybe sex that you had during your period could have caused a pregnancy then you can take a test on the first day that your next period was supposed to start. Good Luck!
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