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What procedure is used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and supplying an electrical voltage to the gel?
agarose gel electrophoresis
If all the bands on an electrophoresis gel are the same color the single stranded DNA sample consisted of one kind of?
false
How does electrophoresis work?
Electrophoresis for nucleic acids (RNA and DNA) works by separating segments by their size. This is possible because RNA and DNA are negatively charged, so will move towards the positive charge applied to one end of the gel. The different segments separate because small fragments of RNA or DNA are able to move more quickly through the gel than larger fragments.
What do DNA bands represent in the agarose gel electrophoresis?
Each band represents a piece of DNA. The extent to which they move through the gel has to do with the fragment's electrophoretic mobility. The lighter the molecule in general the faster it can move through the gel. Usually when performing a gel electrophoresis one would use markers. These markers would be of known molecular weight and would allow you to compare your DNA fragments and find approximate molecular weights.
If people are blood relatives are their DNA similar?
A common way of determining if two people are related to one another is by performing an electrophoresis test. In an electrophoresis test DNA fragments are separated by size as they move through a gel matrix. The more fragments that match in both samples the more likely a person is related to you.
What is the conclusion on the application of gel electrophoresis?
One of the Conclusion of electrophoresis is Visualization of the DNA size. Second is Sequencing the length of DNA of the body.
What procedure is used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and supplying an electrical voltage to the gel?
agarose gel electrophoresis
How does DNA and gel electrophoresis relate?
Gel electrophoresis separates an individual's DNA fragments from one another according to size. An electric current repels a mixture of the negatively-charged DNA fragments through microscopic pores in the gel from the negative to the positive electrode. Upon completion, the separated fragments of DNA can be visualized as a ladder of small bands in the gel by staining with a methylene blue dye solution or smaller DNA segments move more easily through the gel.
One lane of an electrophoresis gel shows a 100bp ladder. What is the purpose of this ladder?
It is used as a marker for molecular weight.
If all the bands on an electrophoresis gel are the same color the single stranded DNA sample consisted of one kind of?
false
How does electrophoresis work?
Electrophoresis for nucleic acids (RNA and DNA) works by separating segments by their size. This is possible because RNA and DNA are negatively charged, so will move towards the positive charge applied to one end of the gel. The different segments separate because small fragments of RNA or DNA are able to move more quickly through the gel than larger fragments.
What is gel electrophoresis and how is it used to analyze?
Gel electrophoresis is a common method to study DNA. It is a very basic way of comparing the mass (mostly size or length of DNA). The main principle behind gel electrophoresis is that DNA has a slight negative charge. When put in a gel (usually agarose gel) DNA will travel through the gel towards a positive charge, which is generated by the electrophoresis machine. The basic idea behind it, is that DNA will travel through the gel towards the positive pole and away the negative pole of the electrophoresis machine. The smaller the fragment, the further it will travel towards the positive pole, as it will go through the gel quickly. The larger fragments will travel slower towards the positive pole, and will travel less compared to the small fragments. This is how one can quickly compare size of DNA fragments, and possibly even compare between 2 or more DNA strands and find similarities.
Why Electrophoresis power supply results in longer gel run time?
There are several factors that control the rate at which a sample moves or migrates in a gel. One of those factors is electric power supply. The larger the voltage applied, the faster the migration. However, there is an upper limit to how much voltage can be applied. If the voltage is too high, it will cause heating in the electrophoresis module and this is turn will negatively affect the integrity of the gel.
What do DNA bands represent in the agarose gel electrophoresis?
Each band represents a piece of DNA. The extent to which they move through the gel has to do with the fragment's electrophoretic mobility. The lighter the molecule in general the faster it can move through the gel. Usually when performing a gel electrophoresis one would use markers. These markers would be of known molecular weight and would allow you to compare your DNA fragments and find approximate molecular weights.
Why is DNA fingerprinting more accurate if the samples are cut with more than one restriction enzyme?
When EcoR1 cuts this DNA, it cuts it at three places into four different segments. EcoR1 is only one of many different restriction enzymes. Each different enzyme cuts DNA at a different site. By using different enzymes, a scientist can cut DNA into many smaller pieces that can be run out on a gel during electrophoresis. Remember that in gel electrophoresis, DNA fragments separate by size. Because these segments have different sizes, they will separate onto a gel at different rates. If different people's DNA is cut by restriction enzymes and then run out on a gel, each person's DNA will leave a different pattern.
How can a mutation that alters a recognition site be detected in gel electrophoresis?
The point mutation has to result in either the removal of a restriction site of the restriction enzymes or the formation of a new one, such that the bands of mutated DNA that form after performing gel electrophoresis are different from the normal one. So a difference in banding patterns would mean that there is a point mutation.
What is the pipet use for?
In one type of experiment, a pipet is used to distribute DNA information. The process is used in Gel Electrophoresis. With the pipet you syphon the DNA material and the chemicals used to bring out the genetic information and you squirt it into the notches in the gel. That is how a pipet is used in one instance.
