When viewing a specimen, you must always use the lower power objective lenses first. Low power lens gives the widest field of view and makes it easier to find the specimen when you look through the microscope. Finding the specimen at high power, without first centering it in the field of view at low power, is nearly impossible.
Whichever objective has the lowest magnification. On many microscopes, it is the 4X or scanning objective.
the las t one it is the best one
- Mandy
the low power lens we first use it
The low power lens was first used
coarse sdjustment knob
Working distance or WD is the distance between each objective and the specimen, when precise focus of the specimen is obtained.
Might add extra, but i really hope this helps! scanning objective - for locating the specimen on the slide (= low power objective) high power objective - magnifies the specimen to provide a detailed image coarse adjustment - used to focus the image when using low power diaphragm - used to adjust the amount of light passing through the specimen revolving nosepiece - holds the three objective lenses - it can be rotated to change the objective in use arm - holds the stage and the lens system - can be used to tilt the microscope (but not advised) substaGe lamp - provides a uniform illumination (more reliable than daylight !!)
low-power because there's more of a field of depth where you can see more rather then in the high power you can't see as much and only one object will be in focus while everything else would be blurry in the background (if there's more then one thing in the slide)
Because it is too hard to find a specimen on high power. Easier to find them and focus them on low power and then increase the magnification and fine-tune the focus to get a better, closer look.
coarse sdjustment knob
Working distance or WD is the distance between each objective and the specimen, when precise focus of the specimen is obtained.
When a very close view of the specimen is necessary. You can focus in on one part of the specimen.
This allows for small adjustments to the focus. Usually the large focus is used with smaller powers (not zoomed in as much), to locate the specimen and move it closer (or further away) from the lens. The fine focus knob is used once the specimen is almost in focus, or if you are on high power.
Try it any other way and you'll see. The scanning objective has the largest field of view, the greatest depth of field, and the greatest working distance. It is the most forgiving objective. It is the easiest to find the specimen and focus on it.
First use the coarse focus followed by the fine focus.
First use the coarse focus followed by the fine focus.
Might add extra, but i really hope this helps! scanning objective - for locating the specimen on the slide (= low power objective) high power objective - magnifies the specimen to provide a detailed image coarse adjustment - used to focus the image when using low power diaphragm - used to adjust the amount of light passing through the specimen revolving nosepiece - holds the three objective lenses - it can be rotated to change the objective in use arm - holds the stage and the lens system - can be used to tilt the microscope (but not advised) substaGe lamp - provides a uniform illumination (more reliable than daylight !!)
You MUST use the coarse focus knob.
A microscope is a scientific instrument with one or more lenses that enhances the view of smaller objects, for example, microorganisms (bacteria) and microsopic materials, which are too small to be seen with the naked eye. The eyepiece lens and objective lenses able to zoom in and out to enlarge and observe the specimens more clearly, with the objective lenses having a much better zoom-in ability. The eyepiece lens is at the top where you look into to observe the specimen. There are usually 3 or 4 objective lenses on a microscope held by a revolving nosepiece/turret, consisting of different times of zoom-in, e.g. 40x, 100x, 400x.... Also, there is a fine focus knob and a coarse focus knob. The coarse focus is for moving the objective lenses nearer/further away from the specimen while the fine focus is for fine-tuning the focus (usually when it is unclear). Use the coarse focus before the fine focus unless there is no need to zoom in on the specimen. Finally, an illuminator might shine a light on the specimen so that you can see it more easily.
low-power because there's more of a field of depth where you can see more rather then in the high power you can't see as much and only one object will be in focus while everything else would be blurry in the background (if there's more then one thing in the slide)
First focus the microscope on the lowest objective using first the coarse djustment knob and then the fine adjustment knob. Once you have it in focus, move on and do the same for the next highest objective. Continue this process until you reach the highest objective and focus it in the same fashion.