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# Suppose you performed a gram stain on a sample from a pure culture of bacteria and observed a friend of red and purple cocci. Adjacent were not always the same color. What do you conclude? # ## The bacteria was not a pure culture.
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∙ 15y ago
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∙ 13y agoThe contrast allows you to differentiate between gram-negative and gram-positive cells by observing the color of the organism.
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Which step is most crucial or most likely to cause poor results in the gram stain?
Gram staining is a type of differential staining in which two types of bacteria are differentiated on the basis of their cell wall either gram positive or gram negative although all the steps in gram staining are crucial, the most important step the most crucial step in the performance of the Gram staining procedure is the decolorization step which is the Acid-Alcohol (3% HCl and 95% Ethanol) and must be timed correctly; the crystal violet stain will be removed from both Gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
Are there endospores in pseudomonas aeruginosa gram staining?
yes
Why is gram staining important in microbiology?
Gram staining is the initial procedure, crutial in determining whether the unknown organism is Gram positive or Gram negative. Once the type is concluded, it is earsier to chose which type of antibiotic will be best to kill the bacterial growth.
What are four possible results of a gram stain?
The four possible results after Gram staining are Gram positive, Gram negative, Gram variable and Gram in-determinant.
Why is gram staining important?
It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.
Why is it important to follow the gram staining procedure?
This is simply important in order to have accurate staining results. If this is not followed, the process of the staining will result to false positives or false negatives.
Why to gram stain bacteria?
gram staining is a biochemical method of identifying bacteria in a more specific way.Thus it is important to differentiate gram positive and gram negative bacteria.
Is gram staining related to flagella?
No. It is a staining on the cell itself.
Who developed Gram staining?
Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Did you stain the bacterial cell wall or the cell cytoplasm?
Yes, it is. Using the Gram staining method, the wall can be seen.
Which step is most crucial or most likely to cause poor results in the gram stain?
Gram staining is a type of differential staining in which two types of bacteria are differentiated on the basis of their cell wall either gram positive or gram negative although all the steps in gram staining are crucial, the most important step the most crucial step in the performance of the Gram staining procedure is the decolorization step which is the Acid-Alcohol (3% HCl and 95% Ethanol) and must be timed correctly; the crystal violet stain will be removed from both Gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).
What is staining for candida albicans?
gram positive
Why is safrinin used in gram staining?
as a couterstain
What is commonly found in a cell membrane?
Peptidoglycan, being the most important element for gram staining, cell differentiation....etc...
