it is selective because only a salt tolerant can grow. 7.5 nacl
it has diffrential properties also. if mannitol formentors turns yellow staphlococus aureus; but if it has no change then its a nonpathogenic staphlococci (s. edermis)
EMB agar is both differential and selective
They can be described as both selective and differential media because they can isolate organisms and partially identify different groups of bacteria
Mannitol is selective for Gram-positive bacteria and differential for mannitol.
Mannitol salt agar is used for the isolation of staphylococci which is found normally on skin (S. aureus). The selectivity is obtained by the high salt concentration that inhibits growth of many groups of bacteria.
media used for oxidase test is non selective media
differential.
Mannitol Salt Agar is selective for staphylococci as the high salt (sodium chloride) levels prohibit most other bacteria from surviving and it is differential as Staphylococci ferment mannitol, producing acid, lowering the pH and turning the media yellow. The development of yellow media presumes the bacteria to be pathogenic Staphylococcus (usually S. aureus). From A Photographic Atlas for the Microbiology Laboratory by Leboffe and Pierce.
Differential media or selective media is an agar that has certain nutrient to grow certain microbes. Simple media is an agar that has simple sugars that many normal microbes thrive in.
Mannitol salt agar is used for the isolation of staphylococci which is found normally on skin (S. aureus). The selectivity is obtained by the high salt concentration that inhibits growth of many groups of bacteria.
its a selective media
media used for oxidase test is non selective media
differential.
Scientists in a lab use selective media and differential media to identify or isolate organisms. Selective media allows only certain types of organisms to grow.
Mannitol Salt Agar is selective for staphylococci as the high salt (sodium chloride) levels prohibit most other bacteria from surviving and it is differential as Staphylococci ferment mannitol, producing acid, lowering the pH and turning the media yellow. The development of yellow media presumes the bacteria to be pathogenic Staphylococcus (usually S. aureus). From A Photographic Atlas for the Microbiology Laboratory by Leboffe and Pierce.
Mannitol Salts agar is a selective and differential media used to identify Staphylococcus sp. The media is selective due to the high salt concentration which prevents all but halophiles from growing. The media is differential due to the presence of mannitol and the indicator Phenol Red. Staphylococcus aureus can ferment mannitol and produce lactic acid as a result. The media contains an indicator called Phenol Red which turns yellow in low pH environments. This results in a yellow halo around the S.aureus colonies. Staphylococcus epidermidis cannot ferment mannitol and so the colour of the media around these colonies does not change. Certain vibro species and other halophiles may grow on these plates some of these appear pink and some may be able to ferment mannitol leading to false positives. S.epidermidis is a normal commensal organism and grows on the skin. S.aureus is a potential pathogen, antibiotic strains exist and can be hospital accquired infections. Search for "MRSA" for more information.
what do you mean? I would say yes. The main types of culture media are Enriched media Differential media Selective media Characterisation media.
Differential media or selective media is an agar that has certain nutrient to grow certain microbes. Simple media is an agar that has simple sugars that many normal microbes thrive in.
It is a SELECTIVE DIFFERENTIAL media used for the groth of Corynebacterium diphtheria.
Mannitol Salt Agar (MSA) is a selective/differential media that inhibits the growth of gram negative bacteria. Only a few bacteria can tolerate the 7.5% NaCl found within this media. The high salt content selects for Staphylococcus and a few salt tolerant enterococci. The presence of the sugar mannitol gives this media its differential properties. When mannitol is fermented (utilized by the bacteria) microorganisms release acidic byproducts that change the pH of the surrounding media. The pH change is indicated by a change in the pH indicator (phenol red) from pink to yellow.
Selective media favor the growth of particular microorganisms. Bile salt or dye like basic fuschin and crystal violet favor the growth of gram-negative bacteria by inhibiting the growth of gram positive bacteria. Maconkey agar is widely used to grow E.coli and related bacteria. Differential media are media that distinguish among different groups of microbes and even permit tentative identification of microorganisms based on their biological characterstics. Blood agar is used to culture of bacteria from human throat. Mckoney agar is both differential and selective media.