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Towards the centre of the chromatogram. The smaller molecules are more mobile and appear towards the outside of the chromatogram.
During the development of a chromatogram, it is better to cover it with a beaker to prevent evaporation, which will affect the movement of the solute and solvent system. This will ensure that the chromatogram will develop properly.
Two ways of identifying amino acids on a chromatogram are paper and gas.
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The chromatogram, or chromatography paper has solvent on it. Each pigment will separate itself from the others due to the relative solubility of that particular pigment.
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By adding a specific reagent.
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it is because sweat and other substances especially colored ones may be present there. so it is best not to touch chromatogram papers to aviod contaminating it and getting erroneous results..:) chem.student..:)