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In DNA replication, enzymes (DNA polymerases) work in the 3 prime to 5 prime end, creating the new strand in the 5 prime to 3 prime direction. This is due to their structure- they add bases to preexisting 3 prime anchors.

Of the five carbons on the deoxyribose, the 3 prime is joined to a hydroxyl and the 5 prime is joined to a phosphate group.

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Q: Why do enzymes work in opposite detections in replication?
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