to make the solution basic enough so that NH be prepared for Fmoc protection
DIPEA is tertiary amine used as base, but poor nucleophile. central N atom is surrounded by the two isopropyl groups and has only space to accept the hydrogen atom. Coupling of peptides occurs at slightly basic pH. HBTU converts Fmoc amino acid into active ester in presence of one equivalent of base. For this purpose DIPEA is routinely used.
in gmp peptide synthesis, there are a lot to consider:Synthesis strategy (i.e. solid phase peptide synthesis, solution phase peptide synthesis)â€¢ Protection groups (i.e. Boc-, Fmoc - processes)â€¢ Purification strategy (i.e. preparative HPLC, conventional chromatography, ion exchange, gel chromatography, counter-current distribution)â€¢ Flexibility and upscalingâ€¢ Special customer request (i.e. dedicated production lines)â€¢ Special equipment (i.e. enzymatic unit, electrolysis unit)For finished dosage forms intended for clinical trials or commercial supply, CreativePep offers for its Active Pharmaceutical Ingredients (APIs)â€¢ Formulation developmentâ€¢ Compatibility studiesâ€¢ Sterile filling in cooperation with partnersâ€¢ Releaseâ€¢ Stability studiesI got this info from the website of Creative Peptides (creative-peptides.com). Hope it's useful for you.
Abbreviations A: Adenosine Ade: Adenine ATP: Adenosine triphosphate B: Nucleobase BMF4TPA: Bis(difluoromethylene)triphosphoric acid BMT: Bismethylene triphosphate Boc: Tert-Butyloxycarbonyl Bop: Bis(2-oxo-3-oxazolidinyl)phosphinic BP : Protected nucleobase BTT: 5-Benzylthio-1-H-tetrazole C: Cytosine CDI: Carbodiimidazole CE: β-Cyanoethyl CEM: Cyanooxymethyl CMPT: N-(cyanomethyl)pyrrolidinium triflate CPG: Controlled pore glass CTP: Cytidine triphosphate Cyt: Cytidine DBU: 1,8-Diazabicyclo[5.4.0]undec-7-ene DCA: Dichloroacetic acid DCI: 4,5-Dicyanoimidazole DEAE: Diethylaminoethyl DIAD: Diisopropyl azodicarboxylate DIPEA: Diisopropylethylamine DMAN: 1,8-Bis-(dimethylamino)naphthalene DMF: N,N-dimethylformamide DMS: Dimethylsulfide DMTr: 4,4′-Dimethoyxltrityl DTD: N,N-dimethylthiuram disulfide EC50 : Half maximal effective concentration EDC/EDCI: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide ETT: 5-(Ethylthio)-1H-tetrazole Fm: 9-Fluorenylmethyl Fmoc: Fluorenylmethyloxycarbonyl G: Guanosine Gua: Guanine IC50 : Half maximal inhibitor concentration IEX-HPLC: Ion-exchange high performance liquid chromatography KHMDS: Hexamethyldisilazide LTMPA: Lithium 2,2,6,6-tetramethylpiperidine amide NHS: N-hydroxysuccinimide NMP: Nucleoside monophosphate Npn : Nucleoside polyphosphate NpnN: Dinucleotide polyphosphate NPP: Nucleotide pyrophosphatase/phosphodiesterase Ns: Nosyl NTP: Nucleoside triphosphate NTP: Nucleoside triphosphate Nuc: Nucleotide or nucleoside ODN: Oligodeoxynucleotides ORN: Oligoribonucleotide OTP: Oxathiaphospholane PEP: Phosphoenolpyruvate Pip: Piperidine PK: Pyruvate dinase ppGpp: Guanosin-3′,5′-bispyrophosphate ppp: RNA 5′-triphosphate RNAs PRR: Pattern recognition receptors Py: Pyridine RP18: Reverse phase C18 RSH: RelA-SpoT homolog SAX: Strong anion exchange T: Thymine TBAF: Tetrabutylammonium fluoride TBHP: tert-Butylhydroperoxide TBS: tert-Butyldimethylsilyl TEA: Triethylamine TEAB: Triethylammonium bicarbonate Tf: Trifluoromethylsulfonyl THF: Tetrahydrofuran Thy: Thymidine TMS: Trimethylsilyl Tr: 2,4,6-Triisopropylbenzenesulfonyl Ts: p-Toluenesulfonyl U: Uridine Ura: Uracil UTP: Uridine triphosphate
A sodium carbonate solution, or any solution for that matter, is a mixture.
A 1M solution of sodium carbonate contains 1 gram formula mass of sodium carbonate dissolved in each liter of solution.
The chemical formula of sodium carbonate is Na2CO3. A solution hasn't a formula.
Sodium carbonate would be deposited at the bottom of the solution.
Barium carbonate will precipitate, sodium nitrate stays in solution (as sodium and nitrate ions)
Sodium carbonate x water --> sodium carbonate (in solution)--> Sodium Carbonate Decahydrate
Sodium carbonate is added to increase the pH of the solution.
it is a transparent solution
when their solution are mixed iron carbonate solid and solution of sodium sulphate is produced..
Sodium hydrogen carbonate is NaHCO3(aq)
Calcium carbonate is not soluble in water, sodium carbonate is soluble in water. Dissolve the mixture and filter: the Na2CO3 pass the filter as a solution and CaCO3 remain on the filter. Gently warm the solution to obtain crystallized sodium carbonate.
take 7.5 gram of sodium carbonate and dissovled in 100 ml of water..thats it.. this solution is called 7.5% sodium carbonate soultion.
Aqueous solution of sodium carbonate has pH between 10-11
When calcium is added in aqueous solution of sodium carbonate white precipitates of calcium carbonate are produced.
Sodium chloride solution with zinc carbonate precipitate.
Sodium Carbonate is not a gas it is a solution. I think you mean how can you test for Carbon Dioxide - bubble it though limewater and you will get Sodium Carbonate.
Water reacts with carbon dioxide in the air to form small amounts of carbonic acid. In a sodium hydroxide solution, this reacts again to form sodium carbonate.
you have to filter the mixture and the solid is copper carbonate
Sodium carbonate is a strong alkaline substance with a pH of 11.6 for a 0.1 M aqueous solution.
Sodium hydrogen carbonate is NaHCO3. It is slightly basic.
Sodium carbonate can dissolve in water to form an aqueous solution, but it can also form a solid salt.